Rabbit anti-Human, Mouse DFFB Polyclonal Antibody | anti-DFFB antibody

DFFB (DNA Fragmentation Factor Subunit beta, Caspase-activated Deoxyribonuclease, CAD, Caspase-activated DNase, Caspase-activated Nuclease, CPAN, DNA Fragmentation Factor 40kD Subunit, DFF-40, CAD, DFF2, DFF40) (FITC)

Cat. Num. AAA6376054

• Gene Names: DFFB; CAD; CPAN; DFF2; DFF40; DFF-40
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DFFB; Polyclonal Antibody; DFFB (DNA Fragmentation Factor Subunit beta; Caspase-activated Deoxyribonuclease; CAD; Caspase-activated DNase; Caspase-activated Nuclease; CPAN; DNA Fragmentation Factor 40kD Subunit; DFF-40; DFF2; DFF40) (FITC); anti-DFFB antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DFFB. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DFFB antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human DFFB, aa1-338 (NP_004393.1).
• Immunogen Sequence: MLQKPKSVKLRALRSPRKFGVAGRSCQEVLRKGCLRFQLPERGSRLCLYEDGTELTEDYFPSVPDNAELVLLTLGQAWQGYVSDIRRFLSAFHEPQVGLIQAAQQLLCDEQAPQRQRLLADLLHNVSQNIAAETRAEDPPWFEGLESRFQSKSGYLRYSCESRIRSYLREVSSYPSTVGAEAQEEFLRVLGSMCQRLRSMQYNGSYFDRGAKGGSRLCTPEGWFSCQGPFDMDSCLSRHSINPYSNRESRILFSTWNLDHIIEKKRTIIPTLVEAIKEQDGREVDWEYFYGLLFTSENLKLVHIVCHKKTTHKLNCDPSRIYKPQTRLKRKQPVRKRQ
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DFFB rabbit polyclonal antibody. Western Blot analysis of DFFB expression in mouse liver.)

Western Blot (WB)

(Western Blot analysis of DFFB expression in transfected 293T cell line by DFFB polyclonal antibody. Lane 1: DFFB transfected lysate (39.1kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-DFFB antibody

Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology.

Product Categories/Family for anti-DFFB antibody

Antibodies; Enzymes; Deoxyribonuclease

NCBI and Uniprot Product Information

• NCBI GI #: 544063487
• NCBI GeneID: 1677
• NCBI Accession #: NP_004393.1
• NCBI GenBank Nucleotide #: NM_004402
• Molecular Weight: 11,482 Da
• NCBI Official Full Name: Homo sapiens DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) (DFFB), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
• NCBI Official Symbol: DFFB
• NCBI Official Synonym Symbols: CAD; CPAN; DFF2; DFF40; DFF-40
• NCBI Protein Information: DNA fragmentation factor subunit beta; DNA fragmentation factor 40 kDa subunit; caspase-activated DNase; caspase-activated deoxyribonuclease; caspase-activated nuclease
• Protein Family: DNA fragmentation factor

NCBI Description

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]