Williams' Medium E w/L-Glutamine w/o Glucose Culture Media
Williams' Medium E w/L-Glutamine w/o Glucose (Powder)
Purity
Cell Culture Grade
Synonyms
Williams' Medium E w/L-Glutamine w/o Glucose; Williams' Medium E w/L-Glutamine w/o Glucose (Powder); Williams' Medium E w/L-Glutamine w/o Glucose culture media
Purity/Purification
Cell Culture Grade
Appearance
Light orange, homogeneous, free flowing powder
Endotoxin Level
< 1EU/ml
pH
4.3
Solubility
Yellow, clear, complete
Directions per Liter
Dissolve 8.8g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Media Formulation
Components shown as g/liter
Inorganic Salts
Calcium Chloride*2H2O 0.265
Cupric Sulfate*H2O 0.0000001
Ferric Nitrate*9H2O 0.0000001
Magnesium Sulfate 0.0977
Manganese Chloride*4H2O 0.0000001
Potassium Chloride 0.4
Sodium Chloride 6.8
Sodium Phosphate Monobasic 0.122
Zinc Sulfate*7H2O 0.0000002
Amino Acids
L-Alanine 0.09
L-Arginine 0.05
L-Asparagine*H2O 0.02
L-Aspartic Acid 0.03
L-Cysteine (free acid) 0.04
L-Cystine*2HCl 0.02
L-Glutamic Acid 0.0445
L-Glutamine 0.292
Glycine 0.05
L-Histidine 0.015
L-Isoleucine 0.05
L-Leucine 0.075
L-Lysine*HCl 0.08746
L-Methionine 0.015
L-Phenylalanine 0.025
L-Proline 0.03
L-Serine 0.01
L-Threonine 0.04
L-Tryptophan 0.01
L-Tyrosine*2Na*2H2O 0.05045
L-Valine 0.05
Vitamins
Ascorbic Acid*Na 0.00227
D-Biotin 0.0005
Choline Chloride 0.0015
Vitamin B-12 0.0002
Calciferol 0.0001
Folic Acid 0.001
myo-Inositol 0.002
Menadione (sodium bisulfate) 0.00001
Niacinamide 0.001
D-Pantothenic Acid*Ca 0.001
Pyridoxal*HCl 0.001
Riboflavin 0.0001
Thiamine*HCl 0.001
DL-a-Tocopherol Phosphate*2Na 0.00001
Retinol Acetate 0.0001
Other
D-Glucose Absent
Glutathione 0.00005
Methyl Linoleate 0.00003
Phenol Red, Sodium 0.0107
Pyruvic Acid, Sodium 0.025
Total: 8.8gL
Inorganic Salts
Calcium Chloride*2H2O 0.265
Cupric Sulfate*H2O 0.0000001
Ferric Nitrate*9H2O 0.0000001
Magnesium Sulfate 0.0977
Manganese Chloride*4H2O 0.0000001
Potassium Chloride 0.4
Sodium Chloride 6.8
Sodium Phosphate Monobasic 0.122
Zinc Sulfate*7H2O 0.0000002
Amino Acids
L-Alanine 0.09
L-Arginine 0.05
L-Asparagine*H2O 0.02
L-Aspartic Acid 0.03
L-Cysteine (free acid) 0.04
L-Cystine*2HCl 0.02
L-Glutamic Acid 0.0445
L-Glutamine 0.292
Glycine 0.05
L-Histidine 0.015
L-Isoleucine 0.05
L-Leucine 0.075
L-Lysine*HCl 0.08746
L-Methionine 0.015
L-Phenylalanine 0.025
L-Proline 0.03
L-Serine 0.01
L-Threonine 0.04
L-Tryptophan 0.01
L-Tyrosine*2Na*2H2O 0.05045
L-Valine 0.05
Vitamins
Ascorbic Acid*Na 0.00227
D-Biotin 0.0005
Choline Chloride 0.0015
Vitamin B-12 0.0002
Calciferol 0.0001
Folic Acid 0.001
myo-Inositol 0.002
Menadione (sodium bisulfate) 0.00001
Niacinamide 0.001
D-Pantothenic Acid*Ca 0.001
Pyridoxal*HCl 0.001
Riboflavin 0.0001
Thiamine*HCl 0.001
DL-a-Tocopherol Phosphate*2Na 0.00001
Retinol Acetate 0.0001
Other
D-Glucose Absent
Glutathione 0.00005
Methyl Linoleate 0.00003
Phenol Red, Sodium 0.0107
Pyruvic Acid, Sodium 0.025
Total: 8.8gL
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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Product Notes
The Williams' Medium E w/L-Glutamine w/o Glucose (Catalog #AAA653064) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Williams' Medium E w/L-Glutamine w/o Glucose, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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