Trypsin 1:250, 0.25% EDTA Culture Media
Trypsin 1:250, 0.25% EDTA (1mM) w/Hanks' Salts, w/o Magnesium & Calcium (Powder)
Purity
Cell Culture Grade
Synonyms
Trypsin 1:250; 0.25% EDTA; 0.25% EDTA (1mM) w/Hanks' Salts; w/o Magnesium & Calcium (Powder); 0.25% EDTA culture media
Purity/Purification
Cell Culture Grade
Form/Format
Light orange, homogeneous, free flowing powder
pH
5.3
Solubility
Light orange, clear, complete
Directions per Liter
Dissolve 12.4 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).
Components in mg/L
EDTA Na2 2H2O: 372.20
Dextrose: 1000.00
Phenol Red, Na: 17.00
Potassium Chloride: 400.00
Potassium Phosphate Monobasic: 60.00
Sodium Chloride: 8000.00
Sodium Phosphate Dibasic: 47.50
Trypsin: 1:250 2500.00
Total: 12.4g/L
Dextrose: 1000.00
Phenol Red, Na: 17.00
Potassium Chloride: 400.00
Potassium Phosphate Monobasic: 60.00
Sodium Chloride: 8000.00
Sodium Phosphate Dibasic: 47.50
Trypsin: 1:250 2500.00
Total: 12.4g/L
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Related Product Information for Trypsin 1:250, 0.25% EDTA culture media
Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. Trypsin is inhibited by organophosphorous compounds such as diisopropylfluorophosphate and porcine-derived inhibitors. Used in protein sequencing and tissue disassociation.
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Product Notes
The Trypsin 1:250, 0.25% EDTA (Catalog #AAA653453) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Trypsin 1:250, 0.25% EDTA, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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