RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red, Biotin Culture Media
RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red, Biotin (Powder)
Synonyms
RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red; Biotin; Biotin (Powder); Biotin culture media
Endotoxin Level
< 1EU/ml
pH
8.5
Appearance
White, homogenous, free flowing powder
Solubility
Colorless, clear, complete.
Directions per Liter
Dissolve 10.4g in 900ml of ddH2O, stirring until completely solubilized. Do NOT heat. Add 2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH units below the desired pH. Add additional water to bring the solution to final volume. Filter-sterilize using a 0.22um membrane. Aliquot into sterile containers.
Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.
Note: It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.
Components shown as g/liter
Inorganic Salts
Calcium Nitrate 4H2O: 0.1 g/liter
Magnesium Sulfate: 0.04884 g/liter
Potassium Chloride: 0.4 g/liter
Sodium Chloride: 6 g/liter
Sodium Phosphate Dibasic: 0.8 g/liter
Amino Acids
L-Arginine: 0.2 g/liter
L-Asparagine (anhydrous): 0.05 g/liter
L-Aspartic Acid: 0.02 g/liter
L-Cystine•2HCl: 0.0652 g/liter
L-Glutamic Acid: 0.02 g/liter
L-Glutamine: 0.3 g/liter
Glycine: 0.01 g/liter
L-Histidine: 0.015 g/liter
Hydroxy-L-proline: 0.02 g/liter
L-Isoleucine: 0.05 g/liter
L-Leucine: 0.05 g/liter
L-Lysine•HCl: 0.04 g/liter
L-Methionine: 0.015 g/liter
L-Phenylalanine: 0.015 g/liter
L-Proline: 0.02 g/liter
L-Serine: 0.03 g/liter
L-Threonine: 0.02 g/liter
L-Tryptophan: 0.005 g/liter
L-Tyrosine•2Na•2H2O: 0.02883 g/liter
L-Valine: 0.02 g/liter
Vitamins
P-Amino Benzoic Acid: 0.001 g/liter
D-Biotin: Absent g/liter
Choline Chloride: 0.003 g/liter
Vitamin B-12: 0.000005 g/liter
Folic Acid: 0.001 g/liter
myo-Inositol: 0.035 g/liter
Niacinamide: 0.001 g/liter
D-Pantothenic Acid•Ca: 0.00025 g/liter
Pyridoxine•HCl: 0.001 g/liter
Riboflavin: 0.0002 g/liter
Thiamine•HCl: 0.001 g/liter
Other
D-Glucose: 2 g/liter
Glutathione: 0.001 g/liter
Phenol Red: Absent
Total: 10.4 g/Liter
Calcium Nitrate 4H2O: 0.1 g/liter
Magnesium Sulfate: 0.04884 g/liter
Potassium Chloride: 0.4 g/liter
Sodium Chloride: 6 g/liter
Sodium Phosphate Dibasic: 0.8 g/liter
Amino Acids
L-Arginine: 0.2 g/liter
L-Asparagine (anhydrous): 0.05 g/liter
L-Aspartic Acid: 0.02 g/liter
L-Cystine•2HCl: 0.0652 g/liter
L-Glutamic Acid: 0.02 g/liter
L-Glutamine: 0.3 g/liter
Glycine: 0.01 g/liter
L-Histidine: 0.015 g/liter
Hydroxy-L-proline: 0.02 g/liter
L-Isoleucine: 0.05 g/liter
L-Leucine: 0.05 g/liter
L-Lysine•HCl: 0.04 g/liter
L-Methionine: 0.015 g/liter
L-Phenylalanine: 0.015 g/liter
L-Proline: 0.02 g/liter
L-Serine: 0.03 g/liter
L-Threonine: 0.02 g/liter
L-Tryptophan: 0.005 g/liter
L-Tyrosine•2Na•2H2O: 0.02883 g/liter
L-Valine: 0.02 g/liter
Vitamins
P-Amino Benzoic Acid: 0.001 g/liter
D-Biotin: Absent g/liter
Choline Chloride: 0.003 g/liter
Vitamin B-12: 0.000005 g/liter
Folic Acid: 0.001 g/liter
myo-Inositol: 0.035 g/liter
Niacinamide: 0.001 g/liter
D-Pantothenic Acid•Ca: 0.00025 g/liter
Pyridoxine•HCl: 0.001 g/liter
Riboflavin: 0.0002 g/liter
Thiamine•HCl: 0.001 g/liter
Other
D-Glucose: 2 g/liter
Glutathione: 0.001 g/liter
Phenol Red: Absent
Total: 10.4 g/Liter
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
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Product Notes
The RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red, Biotin (Catalog #AAA653376) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RPMI 1640 Medium Modified w/L-Glutamine w/o Phenol Red, Biotin, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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