PET Cell Dissociation Reagent Culture Media
PET Cell Dissociation Reagent (BRFF CD-11)
Purity
Cell Culture Grade
Synonyms
PET Cell Dissociation Reagent; PET Cell Dissociation Reagent (BRFF CD-11); PET Cell Dissociation Reagent culture media
Purity/Purification
Cell Culture Grade
pH
7.45
Conductivity
12.65mS
Trypsin Activity
539 BAEEunits/ml
Sterility Test
No Detection of microbial growth.
Suggested Use
After rinsing the cell monolayers using HBS, add 3ml of ice cold PET dissociation reagent. Do not keep the flask in the incubator. Incubate at room temperature and monitor the monolayer by microscopy (preferably phase contrast). When the cells begin to separate, gently remove most of the PET (without removing the cells) and allow the flask to remain at room temperature. To determine whether the cells are ready to harvest, tap the flask (held in an upright position) firmly by hand.
When the cells float free, resuspend them in cold HBS supplemented with 5% Fetal Bovine Serum to stop the tryptic action. Mix well by pipetting the cell suspension, centrifuge and resuspend the cell pellet in the appropriate complete serum-free medium.
When the cells float free, resuspend them in cold HBS supplemented with 5% Fetal Bovine Serum to stop the tryptic action. Mix well by pipetting the cell suspension, centrifuge and resuspend the cell pellet in the appropriate complete serum-free medium.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
May be stored at 4 degree C for short-term (4-6 weeks). For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70 degree C. Aliquots are stable for 6 months.
Shipping: Ships with Dry Ice.
Shipping: Ships with Dry Ice.
Related Product Information for PET Cell Dissociation Reagent culture media
PET Cell Dissociation Reagent is a cell dissociation mix specially formulated for use with the "Cold Trypsin" method. When used to detach monolayer cultures of epithelial cells from the growth substratum, this reagent will yield more viable cells of monolayers grown in serum-free medium. The solution contains polyvinylpyrrolidone, EGTA and trypsin in a HBS base.
Product Categories/Family for PET Cell Dissociation Reagent culture media
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Product Notes
The PET Cell Dissociation Reagent (Catalog #AAA652773) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PET Cell Dissociation Reagent, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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