Nutrient Mixture Ham's F-12 w/L-Glutamine w/o Methionine, Cysteine Culture Media
Nutrient Mixture Ham's F-12 w/L-Glutamine w/o Methionine, Cysteine (Powder)
Purity
Cell Culture Grade
Synonyms
Nutrient Mixture Ham's F-12 w/L-Glutamine w/o Methionine; Cysteine; Cysteine (Powder); Cysteine culture media
Specificity
Ham’s Nutrient Mixtures were originally developed to support clonal growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of HeLa and mouse L-cells. Both mixtures were formulated for use with or without serum supplementation, depending on the cell type being cultured. Ham’s F-10 has been shown to support the growth of human diploid cells, white blood cells for chromosomal analysis, primary explants of rat, rabbit and chicken tissues. Ham’s F-12 has been used for the growth of primary rat hepatocytes and rat prostate epithelial cells. May be used in combination with hormones and transferrin. For optimum buffering, use in a 5% CO2 atmosphere.
Purity/Purification
Cell Culture Grade
Appearance
Light pink, homogenous, free flowing powder
Solubility
Light pink, clear, complete
pH
8.4
Endotoxin
< 1 EU/ml
Directions per Liter
Dissolve 10.66g in 800-900ml of ddH2O stirring gently until completely solubilized. Add 1.17g/L sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Media Formulation
Components shown as g/L
Inorganic Salts:
Calcium Chloride•2H2O 0.0441
Cupric Chloride•2H2O 0.0000017
Ferrous Chloride 0.0003802
Magnesium Chloride•6H2O 0.123
Potassium Chloride 0.224
Sodium Chloride 7.599
Sodium Phosphate Dibasic 0.14204
Zinc Chloride 0.00040
Amino Acids:
L-Alanine 0.009
L-Arginine•HCl 0.211
L-Asparagine•H2O 0.01501
L-Aspartic Acid 0.0133
L-Cysteine•HCL•H2O Absent
L-Glutamic Acid 0.0147
L-Glutamine 0.146
Glycine 0.00751
L-Histidine•HCl•H2O 0.02096
L-Isoleucine 0.00394
L-Leucine 0.0131
L-Lysine•HCl 0.0365
L-Methionine AbsentvL-Phenylalanine 0.00496
L-Proline 0.0345
L-Serine 0.0105
L-Threonine 0.0119
L-Tryptophan 0.00204
L-Tyrosine•Na2•2H2O 0.00778
L-Valine 0.0117
Vitamins
D-Biotin 0.0000073
Choline Chloride 0.01396
Vitamin B-12 0.00136
Folic Acid 0.00132
myo-Inositol 0.018
Niacinamide 0.000037
D-Pantothenic Acid•Ca 0.00048
Pyridoxine•HCl 0.000062
Riboflavin 0.000038
Thiamine•HCl 0.00034
Other:
Thymidine 0.00073
D-Glucose 1.802
Hypoxanthine 0.00408
Linoleic Acid 0.000084
Thioctic Acid 0.00021
Phenol Red, Sodium 0.0013
Putrescine•2HCl 0.000161
Pyruvic Acid, Sodium 0.11
Total: 10.661g/liter
Inorganic Salts:
Calcium Chloride•2H2O 0.0441
Cupric Chloride•2H2O 0.0000017
Ferrous Chloride 0.0003802
Magnesium Chloride•6H2O 0.123
Potassium Chloride 0.224
Sodium Chloride 7.599
Sodium Phosphate Dibasic 0.14204
Zinc Chloride 0.00040
Amino Acids:
L-Alanine 0.009
L-Arginine•HCl 0.211
L-Asparagine•H2O 0.01501
L-Aspartic Acid 0.0133
L-Cysteine•HCL•H2O Absent
L-Glutamic Acid 0.0147
L-Glutamine 0.146
Glycine 0.00751
L-Histidine•HCl•H2O 0.02096
L-Isoleucine 0.00394
L-Leucine 0.0131
L-Lysine•HCl 0.0365
L-Methionine AbsentvL-Phenylalanine 0.00496
L-Proline 0.0345
L-Serine 0.0105
L-Threonine 0.0119
L-Tryptophan 0.00204
L-Tyrosine•Na2•2H2O 0.00778
L-Valine 0.0117
Vitamins
D-Biotin 0.0000073
Choline Chloride 0.01396
Vitamin B-12 0.00136
Folic Acid 0.00132
myo-Inositol 0.018
Niacinamide 0.000037
D-Pantothenic Acid•Ca 0.00048
Pyridoxine•HCl 0.000062
Riboflavin 0.000038
Thiamine•HCl 0.00034
Other:
Thymidine 0.00073
D-Glucose 1.802
Hypoxanthine 0.00408
Linoleic Acid 0.000084
Thioctic Acid 0.00021
Phenol Red, Sodium 0.0013
Putrescine•2HCl 0.000161
Pyruvic Acid, Sodium 0.11
Total: 10.661g/liter
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment.
Prepared media should be kept at 4°C and used within a short period of time.
Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for Nutrient Mixture Ham's F-12 w/L-Glutamine w/o Methionine, Cysteine culture media
Hams Nutrient Mixtures were originally developed to support clonal growth of several clones of Chinese hamster ovary (CHO) cells, as well as clones of Hela and mouse L-cells/ Both mixtures without serum supplementation depending on the cell type being cultured.
Product Categories/Family for Nutrient Mixture Ham's F-12 w/L-Glutamine w/o Methionine, Cysteine culture media
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Product Notes
The Nutrient Mixture Ham's F-12 w/L-Glutamine w/o Methionine, Cysteine (Catalog #AAA652871) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Nutrient Mixture Ham's F-12 w/L-Glutamine w/o Methionine, Cysteine, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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