MEM Eagle w/o L-Glutamine, Folic Acid, Phenol Red Culture Media
MEM Eagle w/o L-Glutamine, Folic Acid, Phenol Red (Powder)
Synonyms
MEM Eagle w/o L-Glutamine; Folic Acid; Phenol Red; Phenol Red (Powder); Phenol Red culture media
Form/Format
White, free flowing, homogeneous powder
Solubility
Colorless, clear, complete
pH
6.2
Endotoxin
<1EU/ml
Directions per Liter
Dissolve 9.27g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 2.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components mg/liter
Inorganic Salts:
Calcium Chloride•2H2O: 264.90
Magnesium Sulfate: 97.70
Potassium Chloride: 400.00
Sodium Chloride: 6800.00
Sodium Phosphate
Monobasic•H2O: 140.30
Amino Acids:
L-Arginine•HCl: 126.40
L-Cystine: 24.02
L-Glutamine: Absent
L-Histidine•HCl•H2O: 42
L-Isoleucine: 52
L-Leucine: 52
L-Lysine•HCl: 72.5
L-Methionine: 15
L-Phenylalanine: 32
L-Threonine: 48
L-Tryptophan: 10
L-Tyrosine: 36
L-Valine: 46
Vitamins:
Choline Chloride: 1.00
Folic Acid: Absent
i-Inositol: 2.00
Nicotinamide: 1.00
D-Pantothenic Acid•Ca: 1.00
Pyridoxal•HCl: 1.00
Riboflavin: 0.10
Thiamine•HCl: 1.00
Other:
D-Glucose: 1000.00
Phenol Red, Sodium: Absent
Total: 9.266g/L
Calcium Chloride•2H2O: 264.90
Magnesium Sulfate: 97.70
Potassium Chloride: 400.00
Sodium Chloride: 6800.00
Sodium Phosphate
Monobasic•H2O: 140.30
Amino Acids:
L-Arginine•HCl: 126.40
L-Cystine: 24.02
L-Glutamine: Absent
L-Histidine•HCl•H2O: 42
L-Isoleucine: 52
L-Leucine: 52
L-Lysine•HCl: 72.5
L-Methionine: 15
L-Phenylalanine: 32
L-Threonine: 48
L-Tryptophan: 10
L-Tyrosine: 36
L-Valine: 46
Vitamins:
Choline Chloride: 1.00
Folic Acid: Absent
i-Inositol: 2.00
Nicotinamide: 1.00
D-Pantothenic Acid•Ca: 1.00
Pyridoxal•HCl: 1.00
Riboflavin: 0.10
Thiamine•HCl: 1.00
Other:
D-Glucose: 1000.00
Phenol Red, Sodium: Absent
Total: 9.266g/L
Preparation and Storage
Store powdered media at Room Temperature. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.
Related Product Information for MEM Eagle w/o L-Glutamine, Folic Acid, Phenol Red culture media
MEM, which incorporates these modifications, includes higher concentrations of amino acids so that the medium more closely approximates the protein composition of mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks' or Eagles' salts has broadened the usefulness of this medium.
Product Categories/Family for MEM Eagle w/o L-Glutamine, Folic Acid, Phenol Red culture media
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Product Notes
The MEM Eagle w/o L-Glutamine, Folic Acid, Phenol Red (Catalog #AAA652906) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MEM Eagle w/o L-Glutamine, Folic Acid, Phenol Red, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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