Typical Testing Data/Standard Curve (for reference only)
Human C-terminal telopeptide of Collagen alpha-1(III) chain ELISA Kit | CTXIII elisa kit
Human C-terminal telopeptide of Collagen alpha-1(III) chain ELISA Kit
Reactivity
Human
Synonyms
C-terminal telopeptide of Collagen alpha-1(III) chain; Human C-terminal telopeptide of Collagen alpha-1(III) chain ELISA Kit; CTXIII elisa kit
Reactivity
Human
Specificity
This assay recognizes recombinant and natural Human C-terminal telopeptide of Collagen alpha-1(III) chain. No significant cross-reactivity or interference was observed.
Assay Type
Competitive
Detection Range
0.156-10 ng/mL
Sensitivity
0.076ng/mL
Intra-Assay CV
<=8.1%
Inter-Assay CV
<=10.2%
Recovery
107%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CTXIII elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human C-terminal telopeptide of Collagen alpha-1(III) chain concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Principle of the Assay||The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-terminal telopeptide of Collagen alpha-1(III) chain, During the reaction, C-terminal telopeptide of Collagen alpha-1(III) chain in the sample or standard competes with a fixed amount of biotin-labeled C-terminal telopeptide of Collagen alpha-1(III) chain for sites on a pre-coated Monoclonal antibody specific to C-terminal telopeptide of Collagen alpha-1(III) chain. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of C-terminal telopeptide of Collagen alpha-1(III) chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay||The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-terminal telopeptide of Collagen alpha-1(III) chain, During the reaction, C-terminal telopeptide of Collagen alpha-1(III) chain in the sample or standard competes with a fixed amount of biotin-labeled C-terminal telopeptide of Collagen alpha-1(III) chain for sites on a pre-coated Monoclonal antibody specific to C-terminal telopeptide of Collagen alpha-1(III) chain. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of C-terminal telopeptide of Collagen alpha-1(III) chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Product Notes
The Human CTXIII (Catalog #AAA288551) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA288551 ELISA Kit recognizes Human CTXIII. It is sometimes possible for the material contained within the vial of "C-terminal telopeptide of Collagen alpha-1(III) chain, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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