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Western Blot (WB) ((0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat ZAP70 Polyclonal Antibody | anti-ZAP70 antibody

Goat anti-ZAP70 Antibody

Gene Names
ZAP70; SRK; STD; TZK; STCD; ZAP-70
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
ZAP70; Polyclonal Antibody; Goat anti-ZAP70 Antibody; zeta-chain (TCR) associated protein kinase 70kDa; FLJ17670; FLJ17679; SRK; STD; TZK; ZAP-70; 70 kDa zeta-associated protein; syk-related tyrosine kinase; tyrosine-protein kinase ZAP-70; zeta-chain associated protein kinase; 70kD; 70 kDa zeta-associated protein antibody; FLJ17670 antibody; FLJ17679 antibody; SRK antibody; STD antibody; syk-related tyrosine kinase antibody; tyrosine-protein kinase ZAP-70 antibody; TZK antibody; ZAP-70 antibody; zeta-chain (TCR) associated protein kinase 70kDa antibody; 70kD antibody; ZAP70 antibody; anti-ZAP70 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SDGYTPEPARITSPD
Sequence Length
619
Applicable Applications for anti-ZAP70 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 70kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9kDa according to NP_001070.2). Recommended concentration: 0.3-1ug/ml.
Immunoprecipitation: This antibody was deemed fit for IP under native conditions (observations from anonymous customer).
Immunogen
Peptide with sequence C-SDGYTPEPARITSPD, from the internal region of the protein sequence according to NP_001070.2.
Epitope
Internal region
Note
This antibody is expected to recognize isoform 1 (NP_001070.2) only.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((0.3ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,873 Da
NCBI Official Full Name
tyrosine-protein kinase ZAP-70 isoform 1
NCBI Official Synonym Full Names
zeta chain of T cell receptor associated protein kinase 70kDa
NCBI Official Symbol
ZAP70
NCBI Official Synonym Symbols
SRK; STD; TZK; STCD; ZAP-70
NCBI Protein Information
tyrosine-protein kinase ZAP-70
UniProt Protein Name
Tyrosine-protein kinase ZAP-70
Protein Family
UniProt Gene Name
ZAP70
UniProt Synonym Gene Names
SRK
UniProt Entry Name
ZAP70_HUMAN

NCBI Description

This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ZAP70: a tyrosine kinase of the Syk family. Associates with the T-cell antigen receptor zeta-chain after TCR stimulation. Phosphorylated by Src-family kinases following antigen receptor activation. Plays a role in lymphocyte activation.

Protein type: EC 2.7.10.2; Kinase, protein; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Syk family

Chromosomal Location of Human Ortholog: 2q12

Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; immunological synapse; intercellular junction; lipid raft; plasma membrane; T cell receptor complex

Molecular Function: ATP binding; non-membrane spanning protein tyrosine kinase activity; phosphotyrosine binding; protein binding; protein-tyrosine kinase activity; receptor binding

Biological Process: adaptive immune response; B cell activation; B cell receptor signaling pathway; beta selection; immune response; inflammatory response; innate immune response; macrophage activation during immune response; negative thymic T cell selection; neutrophil activation during immune response; peptidyl-tyrosine phosphorylation; positive regulation of alpha-beta T cell differentiation; positive regulation of alpha-beta T cell proliferation; positive regulation of B cell differentiation; positive regulation of calcium-mediated signaling; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; positive regulation of T cell differentiation; positive thymic T cell selection; protein amino acid phosphorylation; T cell activation; T cell differentiation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Selective T-cell Defect

Research Articles on ZAP70

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Product Notes

The ZAP70 zap70 (Catalog #AAA422876) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ZAP70 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's ZAP70 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 70kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9kDa according to NP_001070.2). Recommended concentration: 0.3-1ug/ml. Immunoprecipitation: This antibody was deemed fit for IP under native conditions (observations from anonymous customer). Researchers should empirically determine the suitability of the ZAP70 zap70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SDGYTPEPAR ITSPD. It is sometimes possible for the material contained within the vial of "ZAP70, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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