Mouse Cathepsin H ELISA Kit | CTSH elisa kit

Mouse Cathepsin H (CTSH) ELISA Kit

Cat. Num. AAA280649

• Gene Names: Ctsh; AL022844
• Reactivity: Mouse
• Synonyms: Cathepsin H; Mouse Cathepsin H (CTSH) ELISA Kit; CTSH elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse CTSH. No significant cross-reactivity or interference between Mouse CTSH and analogues was observed.
• Sequence Length: 248

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for CTSH elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CTSH in samples. An antibody specific for CTSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCTSH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CTSH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CTSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 914615962
• NCBI GeneID: 13036
• NCBI Accession #: NP_001299578.1
• NCBI GenBank Nucleotide #: NM_001312649.1
• UniProt Accession #: P49935; Q3UCD6
• Molecular Weight: 37,170 Da
• NCBI Official Full Name: pro-cathepsin H isoform 2
• NCBI Official Synonym Full Names: cathepsin H
• NCBI Official Symbol: Ctsh
• NCBI Official Synonym Symbols: AL022844
• NCBI Protein Information: pro-cathepsin H
• UniProt Protein Name: Pro-cathepsin H
• Protein Family: Pro-cathepsin
• UniProt Gene Name: Ctsh

NCBI Description

This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin H mini, heavy, and light chains. In rat and human, these three chains can associate to form the mature enzyme, which has both aminopeptidase and endopeptidase activities. Homozygous knockout mice for this gene exhibit impaired lung surfactant processing and reduced tumorigenesis in a pancreatic cancer model. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015]

Uniprot Description

CTSH: Important for the overall degradation of proteins in lysosomes. Belongs to the peptidase C1 family.

Protein type: EC 3.4.22.16; Protease

Chromosomal Location of Human Ortholog: 9 E3.1|9 47.4 cM

Cellular Component: acrosomal vesicle; axoneme; cytosol; extracellular space; intracellular membrane-bound organelle; lysosome; outer dense fiber

Molecular Function: aminopeptidase activity; caspase activator activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; HLA-A specific activating MHC class I receptor activity; hydrolase activity; kininogen binding; peptidase activator activity involved in apoptotic process; peptidase activity; protein complex binding; protein self-association; serine-type endopeptidase activity

Biological Process: immune response-regulating signal transduction; membrane protein proteolysis; negative regulation of apoptosis; positive regulation of angiogenesis; positive regulation of cell migration; positive regulation of cell proliferation; protein destabilization; proteolysis; proteolysis involved in cellular protein catabolic process; response to retinoic acid; surfactant homeostasis; T cell mediated cytotoxicity; zymogen activation