Cathepsin B / CTSB Recombinant Protein | CTSB recombinant protein

Rat Cathepsin B / CTSB Protein (His Tag)

Cat. Num. AAA2547276

• Purity: > 95 % as determined by SDS-PAGE.
• Synonyms: Cathepsin B / CTSB; Rat Cathepsin B / CTSB Protein (His Tag); Cathepsin B; CTSB; CTSB recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile 25 mM Tris, 150 mM NaCl, pH 7.5
• Sequence Length: 339

• Application Notes: The recombinant rat Ctsb consists 333 amino acids and predicts a molecular mass of 37.1 kDa.
• Predicted N Terminal: His 18
• Endotoxin: < 1.0 EU per mug protein as determined by the LAL method.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CTSB recombinant protein

Background: Cathepsin B is a papain-family cysteine protease that is normally located in lysosomes, where it is involved in the turnover of proteins and plays various roles in maintaining the normal metabolism of cells. This protease has been implicated in pathological conditions, e.g., tumor progression and arthritis. In disease conditions, increases in the expression of cathepsin B occur at both the gene and protein levels. Cathepsin B is synthesized as a preproenzyme and the primary pathways for its normal trafficking to the lysosome utilize mannose 6-phosphate receptors (MPRs). Mature cathepsin B has the ability to degrade several extracellular matrix components at both neutral and acidic pH and has been implicated in the progression of several human and rodent tumors progression and arthritis. Cathepsin B expression is increased in many human cancers at the mRNA, protein and activity levels. It is also frequently overexpressed in premalignant lesions, an observation that associates this protease with local invasive stages of cancer. Increased expression of cathepsin B in primary cancers, and especially in preneoplastic lesions, suggests that this enzyme might have pro-apoptotic features. Active cathepsin B is also secreted from tumours, a mechanism likely to be facilitated by lysosomal exocytosis or extracellular processing by surface activators. Cathepsin B is localized to caveolae on the tumour surface, where binding to the annexin II heterotetramer occurs. Thus CTSB is suggested as a tumor marker. Additionally, Cathepsin B can degrade extracellular matrix proteins, such as collagen IV and laminin, and can activate the precursor form of urokinase plasminogen activator (uPA), perhaps thereby initiating an extracellular proteolytic cascade.

Description: A DNA sequence encoding the rat Ctsb (NP_072119.2) (Met1-Phe339) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 82830420
• NCBI GeneID: 64529
• NCBI Accession #: NP_072119.2
• NCBI GenBank Nucleotide #: NM_022597.2
• UniProt Accession #: P00787
• Molecular Weight: 37,470 Da
• NCBI Official Full Name: cathepsin B preproprotein
• NCBI Official Synonym Full Names: cathepsin B
• NCBI Official Symbol: Ctsb
• NCBI Protein Information: cathepsin B
• UniProt Protein Name: Cathepsin B
• Protein Family: Cathepsin
• UniProt Gene Name: Ctsb

NCBI Description

lysosomal peptidase that is synthesized as a longer precursor and processed to the mature form [RGD, Feb 2006]

Uniprot Description

CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family.

Protein type: Autophagy; EC 3.4.22.1; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 15p12

Cellular Component: apical plasma membrane; caveola; cell surface; cytoplasm; external side of plasma membrane; extracellular region; extracellular space; intracellular membrane-bound organelle; lysosome; mitochondrion; nucleolus; perinuclear region of cytoplasm; sarcolemma

Molecular Function: collagen binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity; endopeptidase activity; kininogen binding; peptidase activity; peptide binding; protein binding; protein complex binding; protein self-association; proteoglycan binding

Biological Process: autophagy; collagen catabolic process; decidualization; entry of virus into host cell; epithelial cell differentiation; proteolysis; proteolysis involved in cellular protein catabolic process; response to amine stimulus; response to cytokine stimulus; response to ethanol; response to glucose stimulus; response to mechanical stimulus; response to organic cyclic substance; response to peptide hormone stimulus; response to wounding; skeletal muscle development; spermatogenesis