Cathepsin-A Recombinant Protein | CTSA recombinant protein

Recombinant Mouse Cathepsin-A

Cat. Num. AAA146793

• Gene Names: Ctsa; PPCA; Ppgb; AU019505
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Cathepsin-A; Recombinant Mouse Cathepsin-A; CTSA Mouse; Lysosomal protective protein (EC: 3.4.16.5); Carboxypeptidase C; Carboxypeptidase; Cathepsin A; Protective protein cathepsin A; PPCA; Protective protein for beta-galactosidase; Cathepsin-A Mouse Recombinant; CTSA recombinant protein

• Host: Sf9 Insect cells
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: Sterile filtered colorless solution.; CTSA protein solution (0.5mg/ml) contains phosphate buffered saline (pH7.4) and 10% glycerol.
• Sequence: APDQDEIDCLPGLAKQPSFR QYSGYLRASD SKHFHYWFVE SQNDPKNSPV VLWLNGGPGC SSLDGLLTEH GPFLIQPDGVTLEYNPYAWN LIANVLYIES PAGVGFSYSD DKMYVTNDTE VAENNYEALK DFFRLFPEYK DNKLFLTGESYAGIYIPTLA VLVMQDPSMN LQGLAVGNGL ASYEQNDNSL VYFAYYHGLL GNRLWTSLQT HCCAQNKCNFYDNKDPECVN NLLEVSRIVG KSGLNIYNLY APCAGGVPGR HRYEDTLVVQ DFGNIFTRLP LKRRFPEALMRSGDKVRLDP PCTNTTAPSN YLNNPYVRKA LHIPESLPRW DMCNFLVNLQ YRRLYQSMNS QYLKLLSSQKYQILLYNGDV DMACNFMGDE WFVDSLNQKM EVQRRPWLVD YGESGEQVAG FVKECSHITF LTIKGAGHMVPTDKPRAAFT MFSRFLNKEP YVEHHHHHH.
• Sequence Length: 474

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for CTSA recombinant protein

Description: CTSA produced in Sf9 Insect cells is a single, glycosylated polypeptide chain containing 459 amino acids (24-474 a.a.) and having a molecular mass of 52.4kDa (Molecular size on SDS-PAGE will appear at approximately 50-70kDa). CTSA is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Introduction: Cathepsin-A (CTSA) is a protective protein which is crucial for both the activity of beta-galactosidase and neuraminidase, CTSA associates with these enzymes and exerts a protective function required for their stability and activity. The CTSA protein is also a carboxypeptidase and can deamidate tachykinins. CTSA is a component of the lysosomal multienzyme complex along with beta-galactosidase and sialidase Neu1. CTSA is a multicatalytic enzyme with deamidase and esterase in addition to carboxypeptidase activities.

Product Categories/Family for CTSA recombinant protein

Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 84042523
• NCBI GeneID: 19025
• NCBI Accession #: NP_001033581.1
• NCBI GenBank Nucleotide #: NM_001038492.2
• UniProt Accession #: P16675; Q8VEF6
• Molecular Weight: 53,844 Da
• NCBI Official Full Name: lysosomal protective protein isoform b preproprotein
• NCBI Official Synonym Full Names: cathepsin A
• NCBI Official Symbol: Ctsa
• NCBI Official Synonym Symbols: PPCA; Ppgb; AU019505
• NCBI Protein Information: lysosomal protective protein
• UniProt Protein Name: Lysosomal protective protein
• Protein Family: Lysosomal protective protein
• UniProt Gene Name: Ctsa
• UniProt Synonym Gene Names: Ppgb; PPCA

NCBI Description

This gene encodes a glycoprotein with deamidase, esterase and carboxypeptidase activities. The encoded protein associates with and provides a protective function to the lysosomal enzymes beta-galactosidase and neuraminidase. Deficiency of the related gene in humans results in galactosialidosis. The proprotein is processed into two shorter chains. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]

Uniprot Description

CTSA: Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins. Defects in CTSA are the cause of galactosialidosis (GSL). A lysosomal storage disease associated with a combined deficiency of beta-galactosidase and neuraminidase, secondary to a defect in cathepsin A. All patients have clinical manifestations typical of a lysosomal disorder, such as coarse facies, cherry red spots, vertebral changes, foam cells in the bone marrow, and vacuolated lymphocytes. Three phenotypic subtypes are recognized. The early infantile form is associated with fetal hydrops, edema, ascites, visceromegaly, skeletal dysplasia, and early death. The late infantile type is characterized by hepatosplenomegaly, growth retardation, cardiac involvement, and a normal or mildly affected mental state. The juvenile/adult form is characterized by myoclonus, ataxia, angiokeratoma, mental retardation, neurologic deterioration, absence of visceromegaly, and long survival. Belongs to the peptidase S10 family.

Protein type: EC 3.4.16.5; Endoplasmic reticulum; Mitochondrial; Protease

Chromosomal Location of Human Ortholog: 2 H3|2 85.27 cM

Cellular Component: intracellular membrane-bound organelle; lysosomal membrane; membrane; mitochondrion; nucleoplasm

Molecular Function: glycoprotein binding; protein binding; serine carboxypeptidase activity

Biological Process: proteolysis; proteolysis involved in cellular protein catabolic process; regulation of protein stability