CCN2 recombinant protein

Recombinant Human CCN2

Cat. Num. AAA7613064

• Gene Names: CTGF; CCN2; NOV2; HCS24; IGFBP8
• Applications: Western Blot, ELISA
• Purity: Greater than 80% by SDS-PAGE gel analyses
• Synonyms: CCN2; Recombinant Human CCN2; CCN 2; CCN family member 2; Connective tissue growth factor; Ctgf; CTGF_HUMAN; Hcs 24; Hcs24; Hypertrophic chondrocyte specific protein 24; Hypertrophic chondrocyte-specific gene product 24; Hypertrophic chondrocyte-specific protein 24; IBP-8; IGF-binding protein 8; IGFBP-8; IGFBP8; Insulin like Growth Factor Binding Protein 8; Insulin-like growth factor-binding protein 8; MGC102839; NOV 2; CCN2 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 80% by SDS-PAGE gel analyses
• Form/Format: Lyophilized from a 0.2um filtered solution in PBS with 5% trehalose, pH7.4
• Sequence Positions: 60-192

• Applicable Applications for CCN2 recombinant protein: Western Blot (WB), ELISA (EIA)
• Species: Human
• Tag: N-terminal His-IF2DI Tag
• Reconstitution: Centrifuge the vial at 10,000rpm for 1 minute, reconstitute at 200ug/ml in sterile distilled water
• Preparation and Storage: Store at -20 degree C for 12 months as lyophilized; 2-8 degree C for 1 month under sterile conditions after reconstitution

NCBI and Uniprot Product Information

• NCBI GI #: 4503123
• NCBI GeneID: 1490
• NCBI Accession #: NP_001892.1
• NCBI GenBank Nucleotide #: NM_001901.2
• UniProt Accession #: P29279; Q6LCY0; Q96A79; Q96QX2; Q9UDL6; E1P578
• Molecular Weight: 38,091 Da
• NCBI Official Full Name: connective tissue growth factor
• NCBI Official Synonym Full Names: connective tissue growth factor
• NCBI Official Symbol: CTGF
• NCBI Official Synonym Symbols: CCN2; NOV2; HCS24; IGFBP8
• NCBI Protein Information: connective tissue growth factor; IBP-8; IGFBP-8; CCN family member 2; IGF-binding protein 8; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8
• UniProt Protein Name: Connective tissue growth factor
• UniProt Gene Name: CTGF
• UniProt Synonym Gene Names: CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8
• UniProt Entry Name: CTGF_HUMAN

NCBI Description

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]

Uniprot Description

CTGF: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 6q23.1

Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; cis-Golgi network; intracellular membrane-bound organelle; perinuclear region of cytoplasm; plasma membrane; extracellular region; cell cortex; cytosol

Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding; protein binding; growth factor activity; fibronectin binding

Biological Process: response to peptide hormone stimulus; epidermis development; response to anoxia; cell-matrix adhesion; positive regulation of collagen biosynthetic process; positive regulation of caspase activity; positive regulation of JNK cascade; cellular lipid metabolic process; response to estradiol stimulus; cell-cell signaling; positive regulation of stress fiber formation; positive regulation of cell proliferation; response to glucose stimulus; tissue homeostasis; response to wounding; angiogenesis; regulation of cell growth; cell differentiation; cell adhesion; positive regulation of cell activation; cartilage condensation; integrin-mediated signaling pathway; transcription initiation from RNA polymerase II promoter; cell migration; ossification; fibroblast growth factor receptor signaling pathway; organ senescence; response to mineralocorticoid stimulus; response to amino acid stimulus; gene expression; regulation of chondrocyte differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of cell differentiation; lung development