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Immunofluorescence (IF) (Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

Connective Tissue Growth Factor Antibody | anti-CTGF antibody

Goat anti-Connective Tissue Growth Factor Antibody

Gene Names
CTGF; CCN2; NOV2; HCS24; IGFBP8
Reactivity
Mouse
Expected from sequence similarity: Human, Rat, Dog
Applications
ELISA, Immunofluorescence
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
Connective Tissue Growth Factor; Goat anti-Connective Tissue Growth Factor Antibody; Goat Anti-CTGF Antibody; CTGF; CCN2; NOV2; IGFBP8; connective tissue growth factor; anti-CTGF antibody
Ordering
For Research Use Only!
Reactivity
Mouse
Expected from sequence similarity: Human, Rat, Dog
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Sequence Length
349
Applicable Applications for anti-CTGF antibody
Peptide ELISA (EIA), Immunofluorescence (IF)
Application Notes
Pep-ELISA: antibody detection limit dilution 1:1000.
IF: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5ug/ml.
Immunogen Sequence
Peptide with sequence C-SLYYRKMYGDMA, from the C Terminus of the protein sequence according to NP_001892.
Peptide Sequence + C
C-SLYYRKMYGDMA
Epitope
C Terminus
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunofluorescence (IF)

(Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

Immunofluorescence (IF) (Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,205 Da
NCBI Official Full Name
connective tissue growth factor
NCBI Official Synonym Full Names
connective tissue growth factor
NCBI Official Symbol
CTGF
NCBI Official Synonym Symbols
CCN2; NOV2; HCS24; IGFBP8
NCBI Protein Information
connective tissue growth factor
UniProt Protein Name
Connective tissue growth factor
UniProt Gene Name
CTGF
UniProt Synonym Gene Names
CCN2; HCS24; IGFBP8; IBP-8; IGF-binding protein 8; IGFBP-8

NCBI Description

The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq, Nov 2009]

Uniprot Description

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.

Research Articles on CTGF

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Product Notes

The CTGF ctgf (Catalog #AAA423707) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Connective Tissue Growth Factor Antibody reacts with Mouse Expected from sequence similarity: Human, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's Connective Tissue Growth Factor can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunofluorescence (IF). Pep-ELISA: antibody detection limit dilution 1:1000. IF: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 5ug/ml. Researchers should empirically determine the suitability of the CTGF ctgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Connective Tissue Growth Factor, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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