CTD Small Phosphatase 1 Recombinant Protein | CTDSP1 recombinant protein

Recombinant Human CTD Small Phosphatase 1

Cat. Num. AAA145338

• Gene Names: CTDSP1; NIF3; SCP1; NLIIF; NLI-IF
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: CTD Small Phosphatase 1; Recombinant Human CTD Small Phosphatase 1; CTDSP1 Human; CTD Small Phosphatase 1 Human Recombinant; Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1; Nuclear LIM interactor-interacting factor 3; NLI-IF; NLI-interacting factor 3; Small C-terminal domain phosphatase 1; SCP1; Small CTD phosphatase 1; CTDSP1; NIF3; NLIIF; CTDSP1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: CTDSP1 solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 20% glycerol and 0.1M NaCl.; Sterile filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MDSSAVITQI SKEEARGPLR GKGDQKSAAS QKPRSRGILH SLFCCVCRDD GEALPAHSGA PLLVEENGAI PKTPVQYLLP EAKAQDSDKI CVVIDLDETL VHSSFKPVNN ADFIIPVEID GVVHQVYVLK RPHVDEFLQR MGELFECVLF TASLAKYADP VADLLDKWGA FRARLFRESC VFHRGNYVKD LSRLGRDLRR VLILDNSPAS YVFHPDNAVP VASWFDNMSD TELHDLLPFF EQLSRVDDVY SVLRQPRPGS
• Sequence Length: 260

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for CTDSP1 recombinant protein

Description: CTDSP1 produced in E Coli is a single, non-glycosylated polypeptide chain containing 280 amino acids (1-260 a.a.) and having a molecular mass of 31.2kDa.CTDSP1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Introduction: CTDSP1 is a class 2C phosphatase with activity dependent on the conserved DxD motif. CTDSP1 preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. In addition, CTDSP1 negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation.

Product Categories/Family for CTDSP1 recombinant protein

Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 332308973
• NCBI GeneID: 58190
• NCBI Accession #: NP_001193807.1
• NCBI GenBank Nucleotide #: NM_001206878.1
• UniProt Accession #: Q9GZU7; Q7Z5Q3; Q7Z5Q4; C9IYG0
• Molecular Weight: 29,128 Da
• NCBI Official Full Name: carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1 isoform 3
• NCBI Official Synonym Full Names: CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase 1
• NCBI Official Symbol: CTDSP1
• NCBI Official Synonym Symbols: NIF3; SCP1; NLIIF; NLI-IF
• NCBI Protein Information: carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1; NLI-interacting factor 3; nuclear LIM interactor-interacting factor 3; small C-terminal domain phosphatase 1
• UniProt Protein Name: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 1
• Protein Family: Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase
• UniProt Gene Name: CTDSP1
• UniProt Synonym Gene Names: NIF3; NLIIF; SCP1; NLI-IF; NLI-interacting factor 3; SCP1; Small CTD phosphatase 1
• UniProt Entry Name: CTDS1_HUMAN

NCBI Description

This gene encodes a member of the small C-terminal domain phosphatase (SCP) family of nuclear phosphatases. These proteins play a role in transcriptional regulation through specific dephosphorylation of phosphoserine 5 within tandem heptapeptide repeats of the C-terminal domain of RNA polymerase II. The encoded protein plays a role in neuronal gene silencing in non-neuronal cells, and may also inhibit osteoblast differentiation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

CTDSP1: Preferentially catalyzes the dephosphorylation of 'Ser- 5' within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: nucleus

Molecular Function: protein binding; metal ion binding; CTD phosphatase activity

Biological Process: regulation of transcription from RNA polymerase II promoter; negative regulation of neuron differentiation; negative regulation of protein amino acid phosphorylation; protein amino acid dephosphorylation