Human Calcitonin ELISA Kit | CT elisa kit

Human Calcitonin ELISA Kit

Cat. Num. AAA762971

• Gene Names: CALCA; CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
• Reactivity: Human
• Synonyms: Calcitonin; Human Calcitonin ELISA Kit; CT/Calcitonin/Thyrocalcitonin; CT elisa kit

• Reactivity: Human
• Sequence Length: 141

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CT elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human CTantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human CT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human CT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman CTcan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 76880484
• NCBI GeneID: 796
• NCBI Accession #: NP_001029124.1
• NCBI GenBank Nucleotide #: NM_001033952.2
• Molecular Weight: 13,898 Da
• NCBI Official Full Name: calcitonin isoform CT preproprotein
• NCBI Official Synonym Full Names: calcitonin related polypeptide alpha
• NCBI Official Symbol: CALCA
• NCBI Official Synonym Symbols: CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
• NCBI Protein Information: calcitonin; calcitonin gene-related peptide 1
• UniProt Protein Name: Calcitonin
• Protein Family: Calcitonin
• UniProt Gene Name: CALCA
• UniProt Synonym Gene Names: CALC1; CCP
• UniProt Entry Name: CALC_HUMAN

NCBI Description

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]

Uniprot Description

CALCA: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: cell soma; cytoplasm; cytosol; extracellular region; extracellular space; nucleus; terminal button

Molecular Function: calcitonin receptor binding; hormone activity; identical protein binding; protein binding; protein complex binding; receptor binding

Biological Process: activation of protein kinase activity; adenylate cyclase activation; aging; antibacterial humoral response; antifungal humoral response; cell-cell signaling; cellular protein metabolic process; cytosolic calcium ion homeostasis; defense response to Gram-negative bacterium; defense response to Gram-positive bacterium; detection of temperature stimulus involved in sensory perception of pain; elevation of cytosolic calcium ion concentration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); embryo implantation; endothelial cell migration; endothelial cell proliferation; feeding behavior; G-protein coupled receptor internalization; G-protein signaling, adenylate cyclase activating pathway; inflammatory response; innate immune response; leukocyte adhesion; monocyte chemotaxis; negative regulation of blood pressure; negative regulation of bone resorption; negative regulation of neurological process; negative regulation of ossification; negative regulation of osteoclast differentiation; negative regulation of smooth muscle contraction; negative regulation of transcription, DNA-dependent; neuropeptide signaling pathway; positive regulation of adenylate cyclase activity; positive regulation of cAMP biosynthetic process; positive regulation of interleukin-1 alpha production; positive regulation of interleukin-8 production; positive regulation of macrophage differentiation; positive regulation of ossification; positive regulation of vasodilation; protein amino acid phosphorylation; receptor internalization; regulation of blood pressure; regulation of heart rate; regulation of systemic arterial blood pressure by neurological process; response to heat; response to pain; response to yeast; vasculature development; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure