CSTB recombinant protein

CSTB, 1-98aa, Human, His tag, E Coli

Cat. Num. AAA203140

• Gene Names: CSTB; PME; ULD; CST6; EPM1; STFB; CPI-B; EPM1A
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: CSTB; 1-98aa; Human; His tag; E Coli; CystatinB; Cystatin B; Stefin B; PME; CST6; CystatinB CHROW21; CPI B; CYTB; EPM1; Liver thiol proteinase inhibitor; STFB.; CSTB recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 50 mM NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF
• Sequence Length: 98

• Applicable Applications for CSTB recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CSTB recombinant protein

CSTB, also known as Cystatin B is an anti-protease implicated in myoclonus epilepsy, a degenerative disease of the central nervous system. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein is able to form a dimer stabilized by noncovalent forces and is thought to play a role in protecting against the proteases leaking from lysosomes. In cells, CSTB is located in the lysosomes and the cytoplasm, but also in the nucleus. Recombinant CSTB, fused to His-tag, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for CSTB recombinant protein

Neuroscience

References

Turk V, et al (1991) FEBS Lett. 285 (2): 213-9. ; Jarvinen M, et al, (1988) Acta Histochem. 82 (1): 5-18 ;

NCBI and Uniprot Product Information

• NCBI GI #: 4503117
• NCBI GeneID: 1476
• NCBI Accession #: NP_000091
• NCBI GenBank Nucleotide #: NM_000100.3
• UniProt Accession #: P04080
• Molecular Weight: 13kDa (118aa), confirmed by MALDI-TOF.
• NCBI Official Full Name: cystatin-B
• NCBI Official Synonym Full Names: cystatin B
• NCBI Official Symbol: CSTB
• NCBI Official Synonym Symbols: PME; ULD; CST6; EPM1; STFB; CPI-B; EPM1A
• NCBI Protein Information: cystatin-B
• UniProt Protein Name: Cystatin-B
• Protein Family: Cystatin
• UniProt Gene Name: CSTB
• UniProt Synonym Gene Names: CST6; STFB

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). One type of mutation responsible for EPM1 is the expansion in the promoter region of this gene of a CCCCGCCCCGCG repeat from 2-3 copies to 30-78 copies. [provided by RefSeq, Jul 2016]

Uniprot Description

This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.