Rabbit AP1M1 Polyclonal Antibody | anti-AP1M1 antibody

AP1M1 Antibody

Cat. Num. AAA9603416

• Gene Names: AP1M1; AP47; CLTNM; MU-1A; CLAPM2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: AP1M1; Polyclonal Antibody; AP1M1 Antibody; Adaptor protein complex AP-1 mu-1 subunit; Adaptor-related protein complex 1 mu-1 subunit; AP-1 complex subunit mu-1; AP-mu chain family member mu1A; Ap1m1; AP1M1_HUMAN; AP47; CLAPM2; Clathrin adaptor protein AP47; Clathrin assembly protein complex 1 medium chain 1; Clathrin assembly protein complex 1 medium chain; Clathrin assembly protein complex AP1 mu subunit; Clathrin coat assembly protein AP47; Clathrin coat-associated protein AP47; CLTNM; Golgi adaptor AP 1 47 kDa protein; Golgi adaptor HA1/AP1 adaptin mu-1 subunit; HA1 47 kDa subunit; MU 1A; Mu-adaptin 1; Mu1A-adaptin; anti-AP1M1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: AP1M1 antibody detects endogenous levels of AP1M1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 435

• Applicable Applications for anti-AP1M1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: A synthesized peptide derived from human AP1M1.
• Subcellular Location: Golgi Apparatus. Cytoplasmic Vesicle > Clathrin-Coated Vesicle Membrane. Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
• Predicted Cross Reactivity: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
• Similarity: Pig (100%), Zebrafish (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from various samples, using AP1M1 antibody. Lane 1: rat heart treated with blocking peptide. Lane 2: Rat heart; Lane 3: Mouse brain; Lane 4: Hepg2; Lane 5: B16F10; )

Immunohistochemistry (IHC)

(MBS9603416 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Western Blot (WB)

(Western blot analysis of extracts of Hacat cells, using AP1M1 antibody.)

Related Product Information for anti-AP1M1 antibody

Function: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Subunit Structure: Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Interacts with MARCH11 (By similarity). Interacts with HIV-1 Nef. Associates with the AP1(MU)-Nef-MHC-I complex; this complex is required for MHC-I internalization.
Post-translational Modifications: Phosphorylation of membrane-bound AP1M1/AP1M2 increases its affinity for sorting signals.
Similarity: Belongs to the adaptor complexes medium subunit family.

NCBI and Uniprot Product Information

• NCBI GI #: 194473724
• NCBI GeneID: 8907
• NCBI Accession #: NP_001123996.1
• NCBI GenBank Nucleotide #: NM_001130524.1
• UniProt Accession #: Q9BXS5; Q4TTY5
• Molecular Weight: Observed: 49 kDa; Predicted: 49 kDa
• NCBI Official Full Name: AP-1 complex subunit mu-1 isoform 1
• NCBI Official Synonym Full Names: adaptor related protein complex 1 subunit mu 1
• NCBI Official Symbol: AP1M1
• NCBI Official Synonym Symbols: AP47; CLTNM; MU-1A; CLAPM2
• NCBI Protein Information: AP-1 complex subunit mu-1
• UniProt Protein Name: AP-1 complex subunit mu-1
• Protein Family: AP-1 complex
• UniProt Gene Name: AP1M1
• UniProt Synonym Gene Names: CLTNM

NCBI Description

The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.