Granulocyte-Colony Stimulating Factor Recombinant Protein | G-CSF recombinant protein

Human Granulocyte-Colony Stimulating Factor Recombinant

Cat. Num. AAA553207

• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: Granulocyte-Colony Stimulating Factor; Human Granulocyte-Colony Stimulating Factor Recombinant; g-csf; gcsf; Granulocyte Colony Stimulating; G-CSF recombinant protein

• Host: E Coli
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant G-CSF was lyophilized from a 0.2 um filtered PBS solution pH 7.0.
• Sequence: MAGPATQSPM KLMALQLLLW HSALWTVQEA TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCA TYKLCHPEEL VLLGHSLGIP WAPLSSCPSQ ALQLAGCLSQ LHSGLFLYQG LLQALEGISP ELGPTLDTLQ LDVADFATTI WQQMEELGMA PALQPTQGAM PAFASAFQRR AGGVLVASHL QSFLEVSYRV LRHLAQP
• Sequence Length: 207

• Domain: four helix cytokine core
• Host Note: Optimized DNA sequence encoding Human G-CSF mature chain was expressed in Escherichia Coli
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human G-CSF is generated by the proteolytic removal of the signal peptide; and propeptide, the molecule has a calculated molecular mass of approximately 19 kDa.; ; Recombinant GCSF is a disulfide-linked monomer protein consisting of 175 amino acid residue subunits, and migrates as an approximately 19 kDa protein under non-reducing; and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for G-CSF recombinant protein

G-CSF is secreted by monocytes, macrophages, and neutrophils after cell activation. It is produced also by stromal cells, fibroblasts, and endothelial cells. Epithelial carcinomas, acute myeloid leukemia cells and various tumor cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL1 and GM-CSF.Comparison of the primary sequence of G-CSF with those of the two other colony stimulating factors, GM-CSF and M-CSF, shows that the three factors are not related to each other. Murine and human G-CSF show a sequence homology of approximately 70 % at the DNA level and of 72 % at the protein level. The G-CSF receptor,CD114, is expressed on all cells of the neutrophils and granulocytes lineage. It is expressed also in placenta cells, endothelial cells and various carcinoma cell lines.Human G-CSF is active in murine cells and vice versa. G-CSF stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophils and granulocytes lineage in a dose-dependent manner. G-CSF synergises with some other cytokines, including GM-CSF and IL4. GM-CSF and G-CSF are required, for example, to develop neutrophilic colonies in vitro. The concerted action of G-CSF and Epo is required to support the growth of mixed colonies of the early erythroid progenitors. A combination of IL4 with G-CSF has been shown to lead to synergistic suppression of the growth of some human leukemic cell lines.

Product Categories/Family for G-CSF recombinant protein

Cytokines

References

• plerixafor (amd3100) and granulocyte colony-stimulating factor (G-CSF) mobilize different cd34+ cell populations based on global gene and microrna expression signatures .Blood, Sep 2009; 114: 2530 - 2541.
• the role of granulocyte-colony stimulating factor (G-CSF) in the healthy brain: a characterization of G-CSF-deficient mice .J. Neurosci., Sep 2009; 29: 11572 - 11581.
• nfi-a directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls β-globin and G-CSF receptor expression .Blood, Aug 2009; 114: 1753 - 1763.
• silencing of microrna-155 in mice during acute inflammatory response leads to derepression of c/ebp beta and down-regulation of G-CSF .Nucleic Acids Res., Jul 2009; 10.1093/nar/gkp577.
• preoperative weekly cisplatin–epirubicin–paclitaxel with G-CSF support in triple-negative large operable breast cancer .Ann. Onc., Jul 2009; 20: 1185 - 1192.