GM-CSF recombinant protein

GM-CSF Protein (Active)

Cat. Num. AAA355602

• Gene Names: CSF2; GMCSF
• Applications: SDS-Page
• Synonyms: GM-CSF; GM-CSF Protein (Active); GM-CSF recombinant protein

• Host: E Coli
• Form/Format: Lyophilized

• Applicable Applications for GM-CSF recombinant protein: SDS-PAGE
• Dimers: < 1% by SDS-PAGE
• Endotoxin Level: < 0.1 ng/ug (1 EU/ug) by LAL test
• Reconstitution: Reconstitute with sterile 18MΩ-cm H2O.
• Storage Buffer: Each vial contains 10 mM Phosphate buffer and 200 mM NaCl (pH7.0) without preservatives
• Storage Buffer: Each vial contains 10 mM Phosphate buffer and 200 mM NaCl (pH7.0) without preservatives.
• Preparation and Storage: Ship at 4 degree C. Upon receipt, store at -20 degree C or -80 degree C for 2 years. After reconstituting, it can store at 4 degree C for a week, it is recommended to add 0.1% HSA or BSA for long term. Avoid repeated freeze and thaw cycles

Related Product Information for GM-CSF recombinant protein

Recombinant human GM-CSF protein with molecular weight 14.5 ± 1.4 kDa.
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. It is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is used as a medication to stimulate the production of white blood cells following chemotherapy. Recently, it has been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The preliminary results have been promising but GM-CSF is not presently FDA-approved for this purpose.

References

1. Breitbach CJ, et al. (2011). "Intravenous delivery of a multi-mechanistic cancer-targeted oncolytic poxvirus in humans". Nature 477 (7362): 99-102. 2. Korzenik J, Dieckgraefe B, Valentine J, Hausman D, Gilbert M (2005). "Sargramostim for active Crohn's disease". N Engl J Med 352 (21): 2193-201.

NCBI and Uniprot Product Information

• NCBI GI #: 183364
• NCBI GeneID: 1437
• UniProt Accession #: P04141; Q14CE8; Q2VPI8; Q8NFI6
• Molecular Weight: 16,295 Da
• NCBI Official Full Name: GM-CSF
• NCBI Official Synonym Full Names: colony stimulating factor 2 (granulocyte-macrophage)
• NCBI Official Symbol: CSF2
• NCBI Official Synonym Symbols: GMCSF
• NCBI Protein Information: granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim
• UniProt Protein Name: Granulocyte-macrophage colony-stimulating factor
• UniProt Gene Name: CSF2
• UniProt Synonym Gene Names: GMCSF; GM-CSF; CSF
• UniProt Entry Name: CSF2_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.

Subunit structure: Monomer. The signaling GM-CSF receptor complex is a dodecamer of two head-to-head hexamers of two alpha, two beta, and two ligand subunits. Ref.13

Subcellular location: Secreted.

Polymorphism: Variant Ile-117 may be a risk factor for atopic asthma.

Pharmaceutical use: Available under the names Leukine (Immunex) and Leucomax (Novartis). Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Sequence similarities: Belongs to the GM-CSF family.