Cellular Apoptosis Susceptibility Blocking Peptide | CSE1L blocking peptide

Cellular Apoptosis Susceptibility Antibody (C-term) Blocking peptide

• Gene Names: CSE1L; CAS; CSE1; XPO2
• Synonyms: Cellular Apoptosis Susceptibility; Cellular Apoptosis Susceptibility Antibody (C-term) Blocking peptide; Exportin-2; Exp2; Cellular apoptosis susceptibility protein; Chromosome segregation 1-like protein; Importin-alpha re-exporter; CSE1L; CAS; XPO2; CSE1L blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human CSE1L. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 971

• Cellular Location: Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm
• Tissue Location: Highly expressed in proliferating cells.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for CSE1L blocking peptide

Export receptor for importin-alpha. Mediates importin- alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

NCBI and Uniprot Product Information

• NCBI GI #: 62297557
• NCBI GeneID: 1434
• UniProt Accession #: P55060; O75432; Q32M40; Q9H5B7; Q9NTS0; Q9UP98; Q9UP99; Q9UPA0; A3RLL6; B2R5T4; E1P5Y0; F8W904
• Molecular Weight: 103,879 Da
• NCBI Official Full Name: Exportin-2
• NCBI Official Synonym Full Names: chromosome segregation 1 like
• NCBI Official Symbol: CSE1L
• NCBI Official Synonym Symbols: CAS; CSE1; XPO2
• NCBI Protein Information: exportin-2
• UniProt Protein Name: Exportin-2
• UniProt Gene Name: CSE1L
• UniProt Synonym Gene Names: CAS; XPO2; Exp2
• UniProt Entry Name: XPO2_HUMAN

NCBI Description

Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

Exportin-2: Export receptor for importin-alpha. Mediates importin- alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Belongs to the XPO2/CSE1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear export; Karyopherin; Nuclear import

Chromosomal Location of Human Ortholog: 20q13

Cellular Component: cytoplasm; cytosol; membrane; nuclear envelope; nucleoplasm; nucleus

Molecular Function: nuclear export signal receptor activity; protein binding

Biological Process: apoptosis; cell proliferation; protein import into nucleus