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SDS-Page

CRYGN recombinant protein

CRYGN, 1-182aa, Human, His tag, E Coli (Denatured)

Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
CRYGN; 1-182aa; Human; His tag; E Coli (Denatured); Crystallin gamma N; Crystallin; gamma N; gamma-crystallin N; CRYGN recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMAQRSG KITLYEGKHF TGQKLEVFGD CDNFQDRGFM NRVNSIHVES GAWVCFNHPD FRGQQFILEH GDYPDFFRWN SHSDHMGSCR PVGMHGEHFR LEIFEGCNFT GQCLEFLEDS PFLQSRGWVK NCVNTIKVYG DGAAWSPRSF GAEDFQLSSS LQSDQGPEEA TTKPATTQPP FLTANL
Sequence Length
182
Applicable Applications for CRYGN recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.

SDS-Page

SDS-Page
Related Product Information for CRYGN recombinant protein
Crystallin, gamma N, also known as CRYGN, belongs to the Crystallins family. Crystallins are divided into alpha, beta and gamma families, and the beta and gamma-crystallins also comprise a superfamily. Crystallins are the major proteins of the vertebrate eye lens, where they maintain the transparency and refractive index of the lens. The CRYGN is differentially regulated after early development, and are involved in cataract formation as a result of either age-related protein degradation or genetic mutation. Recombinant human CRYGN protein, fused to His-tag at N-terminus, was expressed in E Coli.
Product Categories/Family for CRYGN recombinant protein
References
Srivastava O P., et al. (1998) Curr Eye Res. 17:1074-1081.; Srivastava O P., et al. (1998) Biophys Res Commun. 253:288-294.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23.1 kDa (206aa)
NCBI Official Full Name
gamma-crystallin N isoform a
NCBI Official Synonym Full Names
crystallin gamma N
NCBI Official Symbol
CRYGN
NCBI Protein Information
gamma-crystallin N
UniProt Protein Name
Gamma-crystallin N
Protein Family
UniProt Gene Name
CRYGN

NCBI Description

This gene encodes a member of the crystallin family of proteins that are localized to the refractive structure of vertebrate eye lenses. The protein encoded by this gene is unique in that it has both beta and gamma crystallin protein motifs. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Research Articles on CRYGN

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Product Notes

The CRYGN crygn (Catalog #AAA204724) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's CRYGN can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the CRYGN crygn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMAQRSG KITLYEGKHF TGQKLEVFGD CDNFQDRGFM NRVNSIHVES GAWVCFNHPD FRGQQFILEH GDYPDFFRWN SHSDHMGSCR PVGMHGEHFR LEIFEGCNFT GQCLEFLEDS PFLQSRGWVK NCVNTIKVYG DGAAWSPRSF GAEDFQLSSS LQSDQGPEEA TTKPATTQPP FLTANL. It is sometimes possible for the material contained within the vial of "CRYGN, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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