Crystallin, alpha A Recombinant Protein | CRYAA recombinant protein

Crystallin, alpha A, Recombinant, Human

Cat. Num. AAA635907

• Gene Names: CRYAA; CRYA1; HSPB4; CTRCT9
• Purity: Highly Purified; 95% as determined by RP-HPLC and SDS-PAGE
• Synonyms: Crystallin; alpha A; Recombinant; Human; CRYAA recombinant protein

• Host: E Coli
• Purity/Purification: Highly Purified; 95% as determined by RP-HPLC and SDS-PAGE
• Form/Format: Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 50mM sodium chloride, 1mM EDTA.
• Sequence: MDVTIQHPWF KRTLGPFYPS RLFDQFFGEG LFEYDLLPFL SSTISPYYRQ SLFRTVLDSGISEVRSDRDK FVIFLDVKHF SPEDLTVKVQ DDFVEIHGKH NERQDDHGYI SREFHRRYRLPSNVDQSALS CSLSADGMLT FCGPKIQTGL DATHAERAIP VSREEKPTSA PSS

• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, add 0.1% HSA or BSA, aliquot and store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for CRYAA recombinant protein

Alpha crystallins are composed of two gene products ; alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein(sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. The expression of alpha-A is preferentially restricted to the lens cell.

Recombinant CRYAA produced in E. coli is a single,non-glycosylated polypeptide chain containing 173aa and having a molecular mass of 19.91kD.

Product Categories/Family for CRYAA recombinant protein

Molecular Biology; MB-Crystallins

NCBI and Uniprot Product Information

• NCBI GI #: 109731199
• NCBI GeneID: 1409
• UniProt Accession #: P02489; Q53X53; AAB33370
• Molecular Weight: 19.91kiD
• NCBI Official Full Name: Crystallin, alpha A
• NCBI Official Synonym Full Names: crystallin, alpha A
• NCBI Official Symbol: CRYAA
• NCBI Official Synonym Symbols: CRYA1; HSPB4; CTRCT9
• NCBI Protein Information: alpha-crystallin A chain; crystallin, alpha-1; heat shock protein beta-4; human alphaA-crystallin (CRYA1)
• UniProt Protein Name: Alpha-crystallin A chain
• Protein Family: Alpha-crystallin
• UniProt Gene Name: CRYAA
• UniProt Synonym Gene Names: CRYA1; HSPB4; HspB4
• UniProt Entry Name: CRYAA_HUMAN

NCBI Description

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq, Jul 2008]

Uniprot Description

CRYA1: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP, also known as the HSP20) family. Alpha-A is preferentially restricted to the lens.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; metal ion binding; unfolded protein binding; structural constituent of eye lens

Biological Process: visual perception; negative regulation of intracellular transport; response to stimulus; protein refolding; protein homooligomerization; negative regulation of apoptosis

Disease: Cataract 9, Multiple Types