CRLF2 recombinant protein

CRLF2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8122133

• Gene Names: CRLF2; CRL2; TSLPR; CRLF2Y
• Purity: >95% as determined by SDS-PAGE
• Synonyms: CRLF2; CRLF2 Protein; Human; Recombinant (His Tag); Human CRLF2 Protein (His Tag); CRL2 Protein; CRLF2Y Protein; TSLPR Protein; cytokine receptor-like factor 2; CRLF2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys231

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Gln 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CRLF2 (NP_071431.2)(Met1-Lys231) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CRLF2 recombinant protein

Background: Overexpression of cytokine receptor-like factor 2 (CRLF2) due to chromosomal rearrangement has been observed in acute lymphoblastic leukemia (ALL) and reported to contribute to oncogenesis and unfavorable outcome in ALL. The high CRLF2 expression works with the IKZF1 deletion to drive oncogenesis of ALL and has significance in an integrated prognostic model for adult high-risk ALL. Thymic stromal lymphopoietin (TSLP) stimulates in-vitro proliferation of human fetal B-cell precursors. However, its in-vivo role during normal human B lymphopoiesis is unknown. Genetic alterations that cause overexpression of its receptor component, cytokine receptor-like factor 2 (CRLF2), lead to high-risk B-cell acute lymphoblastic leukemia implicating this signaling pathway in leukemogenesis. CRLF2 over-expression is a poor prognostic marker identifying a subset of HR T-ALL patients that could benefit from alternative therapy, potentially targeting the CRLF2 pathway. Cytokine receptor-like factor 2 (CRLF2) rearrangement is found in approximately 50% of pediatric Ph-like B-cell acute lymphoblastic leukemia (B-ALL), and around 50% of CRLF2 + cases harbor JAK mutations. Cytokine receptor-like factor 2(CRLF2) plays an important role in the development of normal B lymphocytes, which can mediate early B cell proliferation and survival.

NCBI and Uniprot Product Information

• NCBI GI #: 19923096
• NCBI GeneID: 64109
• NCBI Accession #: NP_071431.2
• NCBI GenBank Nucleotide #: NM_022148.3
• Molecular Weight: 29,360 Da
• NCBI Official Full Name: cytokine receptor-like factor 2 isoform 1
• NCBI Official Synonym Full Names: cytokine receptor-like factor 2
• NCBI Official Symbol: CRLF2
• NCBI Official Synonym Symbols: CRL2; TSLPR; CRLF2Y
• NCBI Protein Information: cytokine receptor-like factor 2; IL-XR; TSLP receptor; cytokine receptor CRL2 precusor; thymic stromal lymphopoietin protein receptor; thymic stromal-derived lymphopoietin receptor
• Protein Family: Cytokine receptor-like factor

NCBI Description

This gene encodes a member of the type I cytokine receptor family. The encoded protein is a receptor for thymic stromal lymphopoietin (TSLP). Together with the interleukin 7 receptor (IL7R), the encoded protein and TSLP activate STAT3, STAT5, and JAK2 pathways, which control processes such as cell proliferation and development of the hematopoietic system. Rearrangement of this gene with immunoglobulin heavy chain gene (IGH) on chromosome 14, or with P2Y purinoceptor 8 gene (P2RY8) on the same X or Y chromosomes is associated with B-progenitor acute lymphoblastic leukemia (ALL) and Down syndrome ALL. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]