Rat Cyclic AMP Response Element Binding Protein ELISA Kit | CREB elisa kit

Rat Cyclic AMP Response Element Binding Protein ELISA Kit

Cat. Num. AAA730920

• Gene Names: CREB1; CREB
• Reactivity: Rat
• Synonyms: Cyclic AMP Response Element Binding Protein; Rat Cyclic AMP Response Element Binding Protein ELISA Kit; CREB elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This CREB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CREB. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CREB elisa kit

Principle of the Assay: CREB ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CREB antibody and an CREB-HRP conjugate. The assay sample and buffer are incubated together with CREB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CREB concentration since CREB from samples and CREB-HRP conjugate compete for the anti-CREB antibody binding site. Since the number of sites is limited, as more sites are occupied by CREB from the sample, fewer sites are left to bind CREB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CREB concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 33694240
• NCBI GeneID: 1385
• Molecular Weight: 25,445 Da
• NCBI Official Full Name: CREB
• NCBI Official Synonym Full Names: cAMP responsive element binding protein 1
• NCBI Official Symbol: CREB1
• NCBI Official Synonym Symbols: CREB
• NCBI Protein Information: cyclic AMP-responsive element-binding protein 1; CREB-1; transactivator protein; active transcription factor CREB; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1
• UniProt Protein Name: Cyclic AMP-responsive element-binding protein 1
• Protein Family: Cyclic AMP-responsive element-binding protein
• UniProt Gene Name: CREB1
• UniProt Synonym Gene Names: CREB-1; cAMP-responsive element-binding protein 1
• UniProt Entry Name: CREB1_HUMAN

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CREB: a transcription factor of the leucine zipper family of DNA binding proteins. Binds as a homodimer to the cAMP-responsive element (CRE). Phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Two splice-variant isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q34

Cellular Component: nucleoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; transcription cofactor activity; transcription factor activity

Biological Process: circadian rhythm; transcription from RNA polymerase II promoter; lactation; axon guidance; response to glucagon stimulus; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; positive regulation of multicellular organism growth; toll-like receptor 3 signaling pathway; signal transduction; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; response to organic substance; synaptic transmission; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; response to drug; mitochondrion organization and biogenesis; Notch signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; protein stabilization; secretory granule organization and biogenesis; organelle organization and biogenesis; MyD88-independent toll-like receptor signaling pathway; positive regulation of hormone secretion; positive regulation of lipid biosynthetic process; memory; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; pituitary gland development; phospholipase C activation; toll-like receptor signaling pathway; innate immune response; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; regulation of cell size

Disease: Histiocytoma, Angiomatoid Fibrous