Rabbit anti-Human, Mouse CD71 Polyclonal Antibody | anti-CD71 antibody

CD71 (Phospho-Tyr20) Antibody

Cat. Num. AAA9508503

• Gene Names: TFRC; T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: CD71; Polyclonal Antibody; CD71 (Phospho-Tyr20) Antibody; anti-CD71 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: CD71 (Phospho-Tyr20) antibody detects endogenous levels of CD71 only when phosphorylated at Tyr20.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (Phospho-without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 760

• Applicable Applications for anti-CD71 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:10000
• Target Modification: Phospho
• Modification Sites: H:Y20; M:Y20
• Immunogen: The antiserum was produced against synthesized phospho-peptide derived from human CD71 around the phosphorylation site of Tyr20.
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

ELISA (EIA)

(Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CD71 (Phospho-Tyr20) Antibody)

Western Blot (WB)

(Western blot analysis of lysates from 293 cells treated with PMA, using phospho-CD71 (Phospho-Tyr20) antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 189458819
• NCBI GeneID: 7037
• NCBI Accession #: NP_001121620.1
• NCBI GenBank Nucleotide #: NM_001128148.2
• UniProt Accession #: P02786; Q1HE24; Q59G55; Q9UCN0; Q9UCU5; Q9UDF9; Q9UK21; D3DXB0
• Molecular Weight: 84 kDa
• NCBI Official Full Name: transferrin receptor protein 1 isoform 1
• NCBI Official Synonym Full Names: transferrin receptor
• NCBI Official Symbol: TFRC
• NCBI Official Synonym Symbols: T9; TR; TFR; p90; CD71; TFR1; TRFR; IMD46
• NCBI Protein Information: transferrin receptor protein 1
• UniProt Protein Name: Transferrin receptor protein 1
• UniProt Gene Name: TFRC
• UniProt Synonym Gene Names: TR; TfR; TfR1; Trfr; sTfR

NCBI Description

This gene encodes a cell surface receptor necessary for cellular iron uptake by the process of receptor-mediated endocytosis. This receptor is required for erythropoiesis and neurologic development. Multiple alternatively spliced variants have been identified. [provided by RefSeq, Sep 2015]

Uniprot Description

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).