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Mouse Carnitine O-palmitoyltransferase 1 ELISA Kit | CPT1B elisa kit

Mouse Carnitine O-palmitoyltransferase 1, muscle isoform (CPT1B) ELISA Kit

Gene Names
Cpt1b; Cpt1; Cpti; Cpt1-m; Cpti-m; M-cpti
Reactivity
Mouse
Synonyms
Carnitine O-palmitoyltransferase 1; Mouse Carnitine O-palmitoyltransferase 1; muscle isoform (CPT1B) ELISA Kit; CPT1B elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse CPT1B. No significant cross-reactivity or interference between Mouse CPT1B and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for CPT1B elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate CPT1B in samples. An antibody specific for CPT1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCPT1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPT1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPT1B bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
88,217 Da
NCBI Official Full Name
carnitine O-palmitoyltransferase 1, muscle isoform
NCBI Official Synonym Full Names
carnitine palmitoyltransferase 1b, muscle
NCBI Official Symbol
Cpt1b
NCBI Official Synonym Symbols
Cpt1; Cpti; Cpt1-m; Cpti-m; M-cpti
NCBI Protein Information
carnitine O-palmitoyltransferase 1, muscle isoform
UniProt Protein Name
Carnitine O-palmitoyltransferase 1, muscle isoform
UniProt Gene Name
Cpt1b
UniProt Synonym Gene Names
CPT1-M; CPT I; CPTI-M

Uniprot Description

CPT1B: a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq, Jun 2009]

Protein type: EC 2.3.1.21; Lipid Metabolism - fatty acid; Membrane protein, integral; Membrane protein, multi-pass; Transferase

Chromosomal Location of Human Ortholog: 15 E3|15 44.89 cM

Cellular Component: integral component of membrane; intracellular membrane-bound organelle; membrane; mitochondrial outer membrane; mitochondrion

Molecular Function: carnitine O-palmitoyltransferase activity; transferase activity; transferase activity, transferring acyl groups

Biological Process: fatty acid metabolic process; lipid metabolic process; long-chain fatty acid transport; transport

Research Articles on CPT1B

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Product Notes

The Mouse CPT1B cpt1b (Catalog #AAA283526) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283526 ELISA Kit recognizes Mouse CPT1B. It is sometimes possible for the material contained within the vial of "Carnitine O-palmitoyltransferase 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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