SDS-Page
CPLX3 recombinant protein
CPLX3 Protein, Human, Recombinant (His Tag)
Gene Names
CPLX3; CPXIII; CPX-III; Nbla11589
Purity
>95% as determined by SDS-PAGE
Synonyms
CPLX3; CPLX3 Protein; Human; Recombinant (His Tag); Human CPLX3/Complexin 3 Protein (His Tag); CPX-III Protein; CPXIII Protein; Nbla11589 Protein; complexin 3; CPLX3 recombinant protein
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Lys154
Species
Human
Endotoxin
<1.0EU per ug of the protein as determined by the LAL method
Predicted N Terminal
His
Tag
N-His
Protein Construction
A DNA sequence encoding the human CPLX3 (Q8WVH0)(Met1-Lys154) was expressed with an N-terminal polyhistidine tag.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for CPLX3 recombinant protein
Background: CPLX3, also known as complexin 3, belongs to the complexin/synaphin family. As a SNARE-binding protein, complexin (CPX), can act either as a facilitator or as an inhibitor of membrane fusion, constituting a controversial dilemma. CPX acts sequentially on assembling SNAREpins, first facilitating zippering by nearly doubling the distance at which v- and t-SNAREs can engage and then clamping them into a half-zippered fusion-incompetent state. Specifically, the central helix of CPX allows SNAREs to form this intermediate energetic state at 9-15 nm but not when the bilayers are closer than 9 nm. Stabilizing the activated-clamped state at separations of less than 9 nm requires the accessory helix of CPX, which prevents membrane-proximal assembly of SNAREpins. CPLX3 binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A.
References
Newton-Cheh C. et al., 2009, Nat Genet. 41(6): 666-76.Li F. et al., 2011, Nat Struct Mol Biol. 18 (8): 941-6.Amin N. et al., 2012, Mol Psychiatry. 17 (11): 1116-29.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,557 Da
NCBI Official Full Name
complexin-3
NCBI Official Synonym Full Names
complexin 3
NCBI Official Symbol
CPLX3
NCBI Official Synonym Symbols
CPXIII; CPX-III; Nbla11589
NCBI Protein Information
complexin-3; CPX III; complexin III
UniProt Protein Name
Complexin-3
Protein Family
UniProt Gene Name
CPLX3
UniProt Synonym Gene Names
CPX III
UniProt Entry Name
CPLX3_HUMAN
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Product Notes
The CPLX3 cplx3 (Catalog #AAA8121703) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Lys15 4. It is sometimes possible for the material contained within the vial of "CPLX3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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