RAN blocking peptide

RAN Immunizing Peptide

Cat. Num. AAA428424

• Gene Names: RAN; TC4; Gsp1; ARA24
• Reactivity: Human, Mouse, Rat, Dog, Cow
• Synonyms: RAN; RAN Immunizing Peptide; TC4; ARA24; member RAS oncogene family; Gsp1; OK/SW-cl.81; RanGTPase; guanosine triphosphatase Ran; ras-related nuclear protein; RAN blocking peptide

• Reactivity: Human, Mouse, Rat, Dog, Cow
• Form/Format: 100ug of dried peptide
• Sequence: AAQGEPQVQFKLV-C
• Sequence Length: 216

• Preparation and Storage: Shipped at ambient temperature, store at -20 degree C

NCBI and Uniprot Product Information

• NCBI GI #: 5453555
• NCBI GeneID: 5901
• NCBI Accession #: NP_006316.1
• NCBI GenBank Nucleotide #: NM_006325.4
• Molecular Weight: 24,423 Da
• NCBI Official Full Name: GTP-binding nuclear protein Ran isoform 1
• NCBI Official Synonym Full Names: RAN, member RAS oncogene family
• NCBI Official Symbol: RAN
• NCBI Official Synonym Symbols: TC4; Gsp1; ARA24
• NCBI Protein Information: GTP-binding nuclear protein Ran
• UniProt Protein Name: GTP-binding nuclear protein Ran
• Protein Family: Ranakinin
• UniProt Gene Name: RAN
• UniProt Synonym Gene Names: ARA24
• UniProt Entry Name: RAN_HUMAN

NCBI Description

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq, Jul 2008]

Uniprot Description

RAN: a small GTPase belonging to the RAS superfamily that regulates the nucleo-cytoplasmic traffic through the nuclear pore complex, the condensation of chromatin, spindle assembly and cytokinesis, as well as the organization of non-centrosomal microtubules. A major regulator of mitosis and a potential therapeutic target in cancers expressing higher Ras/MEK/ERK and PI3K/Akt/mTORC1 activities. Required for the import of protein into and RNA export from the nucleus. Cycles between the nucleus and cytoplasm in this process. The Ran guanine nucleotide-exchange factor (RanGEF) is nuclear, promoting the GTP-bound state, while the Ran GTPase activating protein (RanGAP) is cytoplasmic, promoting GDP-bound state. RanGDP is imported into the nucleus by nuclear transport factor-2 (NUTF2), where RanGEF catalyses nucleotide exchange and generates RanGTP. RanGTP then binds to transport factors, such as KPNB1 and CAS, and is exported to the cytoplasm, where RanGAP stimulates GTP hydrolysis. Regulates microtubule polymerization during mitosis; micritubule localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1), a Ran-GEF. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. Regulates the formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. GTP-Ran interacts with survivin at S and M phases of the cell cycle. Ran-survivin complexes play a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Interacts with TERT following the tyrosine phosphorylation of TERT induced by H2O2, inducing nuclear export of TERT. Interacts with MAD2L2. Predominantly nuclear during interphase. Becomes dispersed throughout the cytoplasm during mitosis.

Protein type: G protein; G protein, monomeric; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Cell cycle regulation; G protein, monomeric, Ran; Nuclear export

Chromosomal Location of Human Ortholog: 12q24.3

Cellular Component: centriole; chromatin; cytoplasm; cytosol; male germ cell nucleus; melanosome; membrane; midbody; nuclear pore; nucleolus; nucleoplasm; nucleus; recycling endosome

Molecular Function: androgen receptor binding; chromatin binding; dynein intermediate chain binding; GDP binding; GTP binding; GTPase activity; protein binding; protein complex binding; protein domain specific binding; transcription coactivator activity

Biological Process: actin cytoskeleton organization and biogenesis; androgen receptor signaling pathway; cell division; DNA metabolic process; hippocampus development; mitosis; mitotic spindle organization and biogenesis; positive regulation of protein binding; positive regulation of transcription, DNA-dependent; pre-microRNA export from nucleus; protein export from nucleus; protein import into nucleus, translocation; ribosomal large subunit export from nucleus; ribosomal small subunit export from nucleus; RNA-mediated gene silencing; signal transduction; small GTPase mediated signal transduction; spermatid development; tRNA export from nucleus; viral reproduction