CDK9 Antibody | anti-CDK9 antibody

Goat anti-CDK9 (aa86-98) Antibody

Cat. Num. AAA423820

• Gene Names: CDK9; TAK; C-2k; CTK1; CDC2L4; PITALRE
• Reactivity: Mouse; Expected from sequence similarity: Human, Rat, Dog, Pig, Cow
• Applications: ELISA, Western Blot
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: CDK9; Goat anti-CDK9 (aa86-98) Antibody; cyclin-dependent kinase 9; C-2k; CDC2L4; CTK1; PITALRE; TAK; CDC2-related kinase; cell division cycle 2-like protein kinase 4; cell division protein kinase 9; serine/threonine protein kinase PITALRE; tat-associated kinase complex catalytic subunit; anti-CDK9 antibody

• Reactivity: Mouse; Expected from sequence similarity: Human, Rat, Dog, Pig, Cow
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Sequence Length: 372

• Applicable Applications for anti-CDK9 antibody: Peptide ELISA (EIA), Western Blot (WB)
• Application Notes: Pep-ELISA: antibody detection limit dilution 1:1000.; WB: Approx 40kDa band observed in Mouse Skeletal Muscle lysates. Recommended concentration: 1-3ug/ml.
• Immunogen Sequence: Peptide with sequence RTKASPYNRCKGS, from the internal region of the protein sequence according to NP_001252.1.
• Peptide Sequence + C: RTKASPYNRCKGS
• Epitope: Internal Region
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((2ug/ml) staining of Mouse Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 4502747
• NCBI GeneID: 1025
• NCBI Accession #: NP_001252.1; NP_570930.1
• NCBI GenBank Nucleotide #: NM_001261.3
• UniProt Accession #: P50750; Q5JU24; Q5JU25; Q5U006; Q96TF1
• Molecular Weight: 42.8kDa
• NCBI Official Full Name: cyclin-dependent kinase 9
• NCBI Official Synonym Full Names: cyclin dependent kinase 9
• NCBI Official Symbol: CDK9
• NCBI Official Synonym Symbols: TAK; C-2k; CTK1; CDC2L4; PITALRE
• NCBI Protein Information: cyclin-dependent kinase 9
• UniProt Protein Name: Cyclin-dependent kinase 9
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK9
• UniProt Synonym Gene Names: CDC2L4; TAK

NCBI Description

The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008]

Uniprot Description

Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement. RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription. AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect. The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation.