Coronin 1A Recombinant Protein | CORO1A recombinant protein

Recombinant Coronin 1A (CORO1A)

Cat. Num. AAA2029984

• Gene Names: CORO1A; p57; IMD8; TACO; CLABP; HCORO1; CLIPINA
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Coronin 1A; Recombinant Coronin 1A (CORO1A); CORO1A recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MSRQVVRSSK FRHVFGQPAK ADQCYEDVRV SQTTWDSGFC AVNPKFMALICEASGGGAFL VLPLGKTGRV DKNVPLVCGH TAPVLDIAWC PHNDNVIASGSEDCTVMVWE IPDGGLVLPL REPVVTLEGH TKRVGIVAWH PTAQNVLLSAGCDNVILVWD VGTGAAVLTL GPDVHPDTIY SVDWSRDGAL ICTSCRDKRVRVIEMSRQVVRSSK FRHVFGQPAK ADQCYEDVRV SQTTWDSGFC AVNPKFMALICEASGGGAFL VLPLGKTGRV DKNVPLVCGH TAPVLDIAWC PHNDNVIASGSEDCTVMVWE IPDGGLVLPL REPVVTLEGH TKRVGIVAWH PTAQNVLLSAGCDNVILVWD VGTGAAVLTL GPDVHPDTIY SVDWSRDGAL ICTSCRDKRVRVIE
• Sequence Length: 461

• Applicable Applications for CORO1A recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 25.7kDa
• Accurate Molecular Mass (KD): 26kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and S-tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Met1~Glu204
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 300934762
• NCBI GeneID: 11151
• NCBI Accession #: NP_001180262.1
• NCBI GenBank Nucleotide #: NM_001193333.2
• Molecular Weight: 51,026 Da
• NCBI Official Full Name: coronin-1A
• NCBI Official Synonym Full Names: coronin, actin binding protein, 1A
• NCBI Official Symbol: CORO1A
• NCBI Official Synonym Symbols: p57; IMD8; TACO; CLABP; HCORO1; CLIPINA
• NCBI Protein Information: coronin-1A
• UniProt Protein Name: Coronin-1A
• Protein Family: Coronin
• UniProt Gene Name: CORO1A
• UniProt Synonym Gene Names: CORO1; Clipin-A; TACO
• UniProt Entry Name: COR1A_HUMAN

NCBI Description

This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Alternative splicing results in multiple transcript variants. A related pseudogene has been defined on chromosome 16. [provided by RefSeq, Sep 2010]

Uniprot Description

coronin 1A: May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria- infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. Belongs to the WD repeat coronin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: phagocytic vesicle membrane; cortical actin cytoskeleton; protein complex; membrane; axon; lamellipodium; early endosome; cytoplasm; plasma membrane; immunological synapse; actin filament; intercellular junction; phagocytic vesicle; nucleus; phagocytic cup

Molecular Function: actin filament binding; protein C-terminus binding; protein binding; protein homodimerization activity; cytoskeletal protein binding; phosphoinositide 3-kinase binding

Biological Process: negative regulation of vesicle fusion; leukocyte chemotaxis; actin filament organization; regulation of release of sequestered calcium ion into cytosol; phagocytosis; cell-substrate adhesion; uropod organization and biogenesis; phagolysosome formation; regulation of cell shape; regulation of actin filament polymerization; positive chemotaxis; homeostasis of number of cells within a tissue; negative regulation of actin nucleation; calcium ion transport; innate immune response; positive regulation of T cell proliferation; T cell homeostasis; negative regulation of neuron apoptosis; cell motility; actin cytoskeleton organization and biogenesis; positive regulation of cell migration

Disease: Immunodeficiency 8