Corin Recombinant Protein | CRN recombinant protein

Recombinant Corin (CRN)

Cat. Num. AAA2030130

• Gene Names: CORIN; CRN; ATC2; Lrp4; PEE5; TMPRSS10
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Corin; Recombinant Corin (CRN); CRN recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 4.8 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RNTSACM NITHSQCQML PYHATLTPLL SVVRNMEMEK FLKFFTYLHR LSCYQHIMLF GCTLAFPECI IDGDDSHGLL PCRSFCEAAK EGCESVLGMV NYSWPDFLRC SQFRNQTESS NVSRICFSPQ QENGKQLLCG RGENFLCASG ICIPGKLQCN GYNDCDDWSD EAHCNCSENL FHCHTGKCLN YSLVCDGYDD CGDLSDEQNC DCNPTTEHRC GDGRCIAMEW VCDGDHDCVD KSDEVNCSCH SQGLVECRNG QCIPSTFQCD GDEDCKDGSD EENCSVIQTS CQEGDQRCLY NPCLDSCGGS SLCDPNNSLN NCSQCEPITL ELCMNLPYNS TSYPNYFGHR TQKEASISWE SSLFPALVQT NCYKYLMFFS CTILVPKCDV NTGEHIPPCRALCEHSKERC ESVLGIVGLQ WPEDTDCSQF PEENSDNQTC LMP
• Sequence Length: 1042

• Applicable Applications for CRN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 53.1kDa
• Accurate Molecular Mass (KD): 53kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Arg134~Pro573
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4884872
• NCBI GeneID: 10699
• Molecular Weight: 113,297 Da
• NCBI Official Full Name: corin
• NCBI Official Synonym Full Names: corin, serine peptidase
• NCBI Official Symbol: CORIN
• NCBI Official Synonym Symbols: CRN; ATC2; Lrp4; PEE5; TMPRSS10
• NCBI Protein Information: atrial natriuretic peptide-converting enzyme
• UniProt Protein Name: Atrial natriuretic peptide-converting enzyme
• Protein Family: Protein crooked neck
• UniProt Gene Name: CORIN
• UniProt Synonym Gene Names: CRN; TMPRSS10
• UniProt Entry Name: CORIN_HUMAN

NCBI Description

This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

CORIN: Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide. Defects in CORIN are the cause of pre-eclampsia/eclampsia 5 (PEE5); also known as gestational proteinuric hypertension. Preeclampsia is a pregnancy-associated disease with maternal symptoms but placental origin. Unlike most other human disorders, it impacts 2 individuals, the mother and her child, both of whom can be severely affected. Belongs to the peptidase S1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.21.-; Membrane protein, integral; Protease; Cell surface

Chromosomal Location of Human Ortholog: 4p12

Cellular Component: cell surface; integral to plasma membrane; plasma membrane; extracellular region; integral to membrane

Molecular Function: serine-type endopeptidase activity

Biological Process: regulation of blood pressure; peptide hormone processing; female pregnancy; proteolysis; regulation of systemic arterial blood pressure by atrial natriuretic peptide

Disease: Preeclampsia/eclampsia 5