Collagen Type VIII Alpha 2 (COL8a2) Recombinant Protein | COL8a2 recombinant protein

Recombinant Collagen Type VIII Alpha 2 (COL8a2)

Cat. Num. AAA2009417

• Gene Names: COL8A2; FECD; PPCD; FECD1; PPCD2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Collagen Type VIII Alpha 2 (COL8a2); Recombinant Collagen Type VIII Alpha 2 (COL8a2); COL8a2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-DETGIAGLHL PNGGVEGAVL GKGGKPQFGL GELSAHATPA FTAVLTSPFP ASGMPVKFDR TLYNGHSGYN PATGIFTCPV GGVYYFAYHV HVKGTNVWVA LYKNNVPATY TYDEYKKGYL DQASGGAVLQ LRPNDQVWVQ MPSDQANGLY STEYIHSSFS GFLLCPT
• Sequence Length: 703

• Applicable Applications for COL8a2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Asp537~Thr703 (Accession # P25067) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 32964830
• NCBI GeneID: 1296
• NCBI Accession #: NP_005193.1
• NCBI GenBank Nucleotide #: NM_005202.2
• UniProt Accession #: P25067; Q5JV31; Q8TEJ5
• Molecular Weight: 19.4kDa
• NCBI Official Full Name: collagen alpha-2(VIII) chain
• NCBI Official Synonym Full Names: collagen, type VIII, alpha 2
• NCBI Official Symbol: COL8A2
• NCBI Official Synonym Symbols: FECD; PPCD; FECD1; PPCD2
• NCBI Protein Information: collagen alpha-2(VIII) chain; collagen alpha-2(VIII) chain; endothelial collagen; collagen type VIII alpha 2; collagen VIII, alpha-2 polypeptide; dJ665N4.1 (collagen type VIII alpha 2)
• UniProt Protein Name: Collagen alpha-2(VIII) chain
• UniProt Gene Name: COL8A2
• UniProt Entry Name: CO8A2_HUMAN

NCBI Description

This gene encodes the alpha 2 chain of type VIII collagen. This protein is a major component of the basement membrane of the corneal endothelium and forms homo- or heterotrimers with alpha 1 (VIII) type collagens. Defects in this gene are associated with Fuchs endothelial corneal dystrophy and posterior polymorphous corneal dystrophy type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2014]

Uniprot Description

COL8A2: Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis. Defects in COL8A2 are the cause of corneal dystrophy Fuchs endothelial type 1 (FECD1). It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. Defects in COL8A2 are the cause of posterior polymorphous corneal dystrophy type 2 (PPCD2). PPCD is a rare bilateral familial disorder of the corneal epithelium, and is inherited in a autosomal dominant pattern. The clinical features usually present earlier than FECD, being from birth onwards. The disorder is characterized by alterations of Descemet membrane presenting as vesicles, opacities or band-like lesions on slit- lamp examination and specular microscopy. Affected patient typically are asymptomatic.

Protein type: Extracellular matrix; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p34.2

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; collagen; endoplasmic reticulum lumen; extracellular region; basement membrane

Molecular Function: protein binding, bridging; extracellular matrix structural constituent

Biological Process: collagen catabolic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; epithelial cell proliferation; cell-cell adhesion; camera-type eye morphogenesis; angiogenesis

Disease: Corneal Dystrophy, Posterior Polymorphous, 2; Corneal Dystrophy, Fuchs Endothelial, 1