Human Collagen Type VI Alpha 3 ELISA Kit | COL6alpha3 elisa kit

Human Collagen Type VI Alpha 3 ELISA Kit

Cat. Num. AAA761269

• Gene Names: COL6A3; DYT27; UCMD1; BTHLM1
• Reactivity: Human
• Synonyms: Collagen Type VI Alpha 3; Human Collagen Type VI Alpha 3 ELISA Kit; COL6alpha3/COL6A3/DYT27; COL6alpha3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of COL6alpha3. No significant cross-reactivity or interference between COL6alpha3 and analogues was observed.
• Sequence Length: 3176

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL6alpha3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 3127926
• NCBI GeneID: 1293
• UniProt Accession #: P12111; Q16501; Q53QF4; Q53QF6; A8MT30; B4E3U5; B7ZMJ7; E9PFQ6; E9PGQ9
• Molecular Weight: 134,707 Da
• NCBI Official Full Name: collagen type VI, alpha 3 chain
• NCBI Official Synonym Full Names: collagen type VI alpha 3
• NCBI Official Symbol: COL6A3
• NCBI Official Synonym Symbols: DYT27; UCMD1; BTHLM1
• NCBI Protein Information: collagen alpha-3(VI) chain
• UniProt Protein Name: Collagen alpha-3(VI) chain
• UniProt Gene Name: COL6A3
• UniProt Entry Name: CO6A3_HUMAN

NCBI Description

This gene encodes the alpha-3 chain, one of the three alpha chains of type VI collagen, a beaded filament collagen found in most connective tissues. The alpha-3 chain of type VI collagen is much larger than the alpha-1 and -2 chains. This difference in size is largely due to an increase in the number of subdomains, similar to von Willebrand Factor type A domains, that are found in the amino terminal globular domain of all the alpha chains. These domains have been shown to bind extracellular matrix proteins, an interaction that explains the importance of this collagen in organizing matrix components. Mutations in the type VI collagen genes are associated with Bethlem myopathy, a rare autosomal dominant proximal myopathy with early childhood onset. Mutations in this gene are also a cause of Ullrich congenital muscular dystrophy, also referred to as Ullrich scleroatonic muscular dystrophy, an autosomal recessive congenital myopathy that is more severe than Bethlem myopathy. Multiple transcript variants have been identified, but the full-length nature of only some of these variants has been described. [provided by RefSeq, Jun 2009]

Uniprot Description

Collagen VI acts as a cell-binding protein.