Human Collagen type IV alpha-3-binding protein ELISA Kit | COL4A3BP elisa kit

Human Collagen type IV alpha-3-binding protein ELISA Kit

Cat. Num. AAA763392

• Gene Names: Col4a3bp; CERT; GPBP; AU016711; 2810404O15Rik; 9230101K08Rik
• Reactivity: Human
• Synonyms: Collagen type IV alpha-3-binding protein; Human Collagen type IV alpha-3-binding protein ELISA Kit; COL4A3BP/Collagen type IV alpha-3-binding protein/StAR-related lipid transfer protein 11/START domain-containing protein 11/StARD11/Ceramide transfer protein/hCERT/Goodpasture antigen-binding protein/GPBP//CERT/CERTL/FLJ20597/STARD11; COL4A3BP elisa kit

• Reactivity: Human
• Sequence Length: 598

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for COL4A3BP elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human COL4A3BP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human COL4A3BP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human COL4A3BP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human COL4A3BP can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 255982524
• NCBI GeneID: 68018
• NCBI Accession #: NP_001157694.1
• NCBI GenBank Nucleotide #: NM_001164222.1
• Molecular Weight: 35,929 Da
• NCBI Official Full Name: collagen type IV alpha-3-binding protein isoform 2
• NCBI Official Synonym Full Names: collagen, type IV, alpha 3 (Goodpasture antigen) binding protein
• NCBI Official Symbol: Col4a3bp
• NCBI Official Synonym Symbols: CERT; GPBP; AU016711; 2810404O15Rik; 9230101K08Rik
• NCBI Protein Information: collagen type IV alpha-3-binding protein
• UniProt Protein Name: Collagen type IV alpha-3-binding protein
• Protein Family: Collagen type IV alpha-3-binding protein
• UniProt Gene Name: Col4a3bp
• UniProt Synonym Gene Names: Stard11; GPBP; StARD11
• UniProt Entry Name: C43BP_MOUSE

Uniprot Description

COL4A3BP: a protein with a lipid-binding domain that mediates intracellular trafficking of ceramide in a non-vesicular manner. Implicated in human autoimmune diseases via its association with the Goodpasture antigen. Phosphorylation on Ser-132 decreases the affinity toward phosphatidylinositol 4-phosphate at Golgi membranes and reduces ceramide transfer activity. GPBP has been reported to have Ser/Thr protein kinase activity towards the Goodpasture (GP) antigen, but lacks a conventional serine/threonine kinase domain. Two human isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding; Protein kinase, atypical; Kinase, protein; EC 2.7.11.9; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; COL4A3BP family

Cellular Component: cytoplasm; cytosol; endoplasmic reticulum; Golgi apparatus; mitochondrion; nucleoplasm; perinuclear region of cytoplasm

Molecular Function: kinase activity; lipid binding

Biological Process: cell morphogenesis; cell proliferation; ceramide metabolic process; endoplasmic reticulum organization and biogenesis; heart morphogenesis; in utero embryonic development; lipid homeostasis; lipid transport; muscle contraction; signal transduction; transport