Human Collagen Type IV ELISA Kit | COL4 elisa kit

Human Collagen Type IV ELISA Kit

Cat. Num. AAA764396

• Gene Names: Cg25C; alpha(IV)1/Cg25C; alpha1; cg25c; cg25C; CG25C; CG4145; Cgc25; Cgc25C; Col IV alpha(1); col4a1; Coll; Coll IV; CT12803; Dcg; Dcg1; DCg1; DmelCG4145; gp125
• Reactivity: Human
• Synonyms: Collagen Type IV; Human Collagen Type IV ELISA Kit; COL4/Type-IV Collagen; COL4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of COL4. No significant cross-reactivity or interference between COL4 and analogues was observed.
• Sequence Length: 1779

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL4 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 24581824
• NCBI GeneID: 33727
• NCBI Accession #: NP_723046.1
• NCBI GenBank Nucleotide #: NM_164617.2
• Molecular Weight: 174,300 Da
• NCBI Official Full Name: collagen type IV, isoform C
• NCBI Official Synonym Full Names: Collagen type IV
• NCBI Official Symbol: Cg25C
• NCBI Official Synonym Symbols: alpha(IV)1/Cg25C; alpha1; cg25c; cg25C; CG25C; CG4145; Cgc25; Cgc25C; Col IV alpha(1); col4a1; Coll; Coll IV; CT12803; Dcg; Dcg1; DCg1; DmelCG4145; gp125
• NCBI Protein Information: CG4145 gene product from transcript CG4145-RB
• UniProt Protein Name: Collagen alpha-1(IV) chain
• Protein Family: Collagen type IV alpha-3-binding protein
• UniProt Gene Name: Cg25C
• UniProt Synonym Gene Names: DCg1
• UniProt Entry Name: CO4A1_DROME

Uniprot Description

Collagen type IV is specific for basement membranes.