Human collagen, type III, alpha 1 ELISA Kit | COL3A1 elisa kit

Human Collagen, type III, alpha 1, COL3A1 ELISA Kit

Cat. Num. AAA703611

• Gene Names: COL3A1; EDS4A
• Reactivity: Human
• Synonyms: collagen; type III; alpha 1; Human Collagen; COL3A1 ELISA Kit; alpha 1 (COL3A1) ELISA Kit; EDS4A; FLJ34534; Ehlers-Danlos syndrome type IV; autosomal dominant; alpha1 (III) collagen; collagen type III alpha 1; fetal; COL3A1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human COL3A1. No significant cross-reactivity or interference between human COL3A1 and analogues was observed.
• Sequence Length: 1466

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: < 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COL3A1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COL3A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COL3A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL3A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL3A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4502951
• NCBI GeneID: 1281
• NCBI Accession #: NP_000081.1
• NCBI GenBank Nucleotide #: NM_000090.3
• UniProt Accession #: P02461; P78429; Q15112; Q16403; Q53S91; Q541P8; Q6LDB3; Q6LDJ2; Q6LDJ3; Q7KZ56; D2JYH5; D3DPH4
• Molecular Weight: 138,564 Da
• NCBI Official Full Name: collagen alpha-1(III) chain preproprotein
• NCBI Official Synonym Full Names: collagen, type III, alpha 1
• NCBI Official Symbol: COL3A1
• NCBI Official Synonym Symbols: EDS4A
• NCBI Protein Information: collagen alpha-1(III) chain; collagen alpha-1(III) chain; collagen, fetal; alpha1 (III) collagen; Ehlers-Danlos syndrome type IV, autosomal dominant
• UniProt Protein Name: Collagen alpha-1(III) chain
• Protein Family: Collagen
• UniProt Gene Name: COL3A1
• UniProt Entry Name: CO3A1_HUMAN

NCBI Description

This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]

Uniprot Description

Function: Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.