TAG1/Contactin 2 Recombinant Protein | CNTN2 recombinant protein

TAG1/Contactin 2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120280

• Gene Names: CNTN2; AXT; TAX; TAX1; TAG-1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: TAG1/Contactin 2; TAG1/Contactin 2 Protein; Human; Recombinant (His Tag); Human Contactin 2/CNTN2 Protein (His Tag); AXT Protein; FAME5 Protein; TAG-1 Protein; TAX Protein; TAX1 Protein; contactin 2 (axonal); CNTN2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn1012

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ser 31
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human CNTN2 (NP_005067.1) precursor (Met 1-Asn 1012) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CNTN2 recombinant protein

Background: Contactins are a subgroup of molecules belonging to the immunoglobulin superfamily that are expressed exclusively in the nervous system. The subgroup consists of six members: Contactin-1, Contactin-2 (TAG-1), Contactin-3 (BIG-1), BIG-2, Contactin-5 (NB-2) and NB-3. Since their identification in the late 1980s, Contactin-1 and Contactin-2 have been studied extensively. Axonal expression and the neurite extension activity of Contactin-1 and Contactin-2 attracted researchers to study the function of these molecules in axon guidance during development. Contactin-1 and Contactin-2 have come to be known as the principal molecules in the function and maintenance of myelinated neurons. In contrast, the function of the other four members of this subgroup remained unknown until recently. Contactin-2, also known as CNTN2, is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. The human, rat, and chicken Contactin-2 are alternatively known as TAX1 (transiently-expressed axonal glycoprotein), TAG1 (transient axonal glycoprotein), and axonin-1, respectively. Human Contactin-2 shares approximately 91% and 75% amino acid sequence identity with rat and chicken Contactin-2, respectively. Contactin-2 is expressed by a subset of neuronal populations in the developing central nervous system (CNS) and peripheral nervous system (PNS). Contactin-2 is also expressed by oligodendrocytes and Schwann cells, which are myelinating glial cells of the CNS and PNS, respectively. Contactin-2 may play a role in the formation of axon connections in the developing nervous system. Contactin-2 is also involved in glial tumorigenesis and may provide a potential target for therapeutic intervention. During embryonic development, Contactin-2 interacts either in a homophilic, or heterophilic fashion with various transmembrane proteins.

References

Kyriakopoulou K, et al. (2002) A combination of chain and neurophilic migration involving the adhesion molecule TAG-1 in the caudal medulla. Development 129 (2):287-96.Traka M, et al. (2002) The neuronal adhesion protein TAG-1 is expressed by Schwann cells and oligodendrocytes and is localized to the juxtaparanodal region of myelinated fibers. J Neurosci. 22(8):3016-24.Traka M, et al. (2003) Association of TAG-1 with Caspr2 is essential for the molecular organization of juxtaparanodal regions of myelinated fibers. J Cell Biol. 162(6):1161-72.Soares S, et al. (2005) Neuronal and glial expression of the adhesion molecule TAG-1 is regulated after peripheral nerve lesion or central neurodegeneration of adult nervous system. Eur J Neurosci. 21(5):1169-80.Derfuss T, et al. (2009) Contactin-2/TAG-1-directed autoimmunity is identified in multiple sclerosis patients and mediates gray matter pathology in animals. Proc Natl Acad Sci U S A. 106(20):8302-7.

NCBI and Uniprot Product Information

• NCBI GI #: 4827022
• NCBI GeneID: 6900
• NCBI Accession #: NP_005067.1
• NCBI GenBank Nucleotide #: NM_005076.3
• UniProt Accession #: Q02246; P78432; Q5T054
• Molecular Weight: 113,393 Da
• NCBI Official Full Name: contactin-2
• NCBI Official Synonym Full Names: contactin 2 (axonal)
• NCBI Official Symbol: CNTN2
• NCBI Official Synonym Symbols: AXT; TAX; TAX1; TAG-1
• NCBI Protein Information: contactin-2; TAX-1; axonal glycoprotein TAG-1; axonin-1 cell adhesion molecule; transient axonal glycoprotein 1; contactin 2 (transiently expressed); transiently-expressed axonal glycoprotein
• UniProt Protein Name: Contactin-2
• Protein Family: Contactin
• UniProt Gene Name: CNTN2
• UniProt Synonym Gene Names: AXT; TAG1; TAX1; TAX-1
• UniProt Entry Name: CNTN2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunoglobulin superfamily. It is a glycosylphosphatidylinositol (GPI)-anchored neuronal membrane protein that functions as a cell adhesion molecule. It may play a role in the formation of axon connections in the developing nervous system. It may also be involved in glial tumorigenesis and may provide a potential target for therapeutic intervention. [provided by RefSeq, Jul 2008]

Uniprot Description

CNTN2: May play a role in the initial growth and guidance of axons. May be involved in cell adhesion. Belongs to the immunoglobulin superfamily. Contactin family.

Protein type: Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: voltage-gated potassium channel complex; cell surface; cell soma; integral to plasma membrane; plasma membrane; synapse; myelin sheath

Molecular Function: identical protein binding; protein self-association; carbohydrate binding; glycoprotein binding

Biological Process: axon guidance; cerebral cortex GABAergic interneuron migration; myelination in the central nervous system; positive regulation of adenosine receptor signaling pathway; axonal fasciculation; regulation of astrocyte differentiation; microtubule cytoskeleton organization and biogenesis; learning; adult walking behavior; negative regulation of neuron differentiation; receptor internalization; clustering of voltage-gated potassium channels; regulation of axon diameter; cell adhesion; regulation of neuronal synaptic plasticity

Disease: Epilepsy, Familial Adult Myoclonic, 5