CNPY4 recombinant protein

CNPY4 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123243

• Gene Names: Cnpy4; 2610019P18Rik
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CNPY4; CNPY4 Protein; Mouse; Recombinant (His Tag); Mouse CNPY4 Protein (His Tag); 2610019P18Rik Protein; canopy FGF signaling regulator 4; CNPY4 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Leu245

• Species: Mouse
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Glu 28
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse CNPY4 (NP_848727.1)(Met1-Leu245) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CNPY4 recombinant protein

Background: CNPY4 belongs to the canopy family. CNPY4 interacts with toll-like receptor 4 (TLR4) and plays a role in the regulation of the cell surface expression of TLR4. Toll-like receptors (TLRs) recognize microbial products and induce immune responses. Lipopolysaccharide is recognized by the receptor complex consisting of TLR4 and MD-2. As CNPY4, PRAT4B also regulates cell surface expression of TLR4. PRAT4B has a signal peptide followed by a mature peptide. It is associated with the hypoglycosylated, immature form of TLR4 but not with MD-2 or TLR2.

References

Stelzl U, et al. (2005) A human protein-protein interaction network: a resource for annotating the proteome. Cell. 122(6):957-68.Hocking JC, et al. (2010) Distinct roles for Robo2 in the regulation of axon and dendrite growth by retinal ganglion cells. Mech Dev. 127(1-2):36-48.Hart BE, et al. (2012) Cell surface trafficking of TLR1 is differentially regulated by the chaperones PRAT4A and PRAT4B. J Biol Chem. 287(20):16550-62.

NCBI and Uniprot Product Information

• NCBI GI #: 30519939
• NCBI GeneID: 66455
• NCBI Accession #: NP_848727.1
• NCBI GenBank Nucleotide #: NM_178612.4
• UniProt Accession #: Q8BQ47; Q8BT84; Q8BTB1; Q8CCG0
• Molecular Weight: 28,094 Da
• NCBI Official Full Name: protein canopy homolog 4
• NCBI Official Synonym Full Names: canopy 4 homolog (zebrafish)
• NCBI Official Symbol: Cnpy4
• NCBI Official Synonym Symbols: 2610019P18Rik
• NCBI Protein Information: protein canopy homolog 4; protein associated with Tlr4
• UniProt Protein Name: Protein canopy homolog 4
• Protein Family: Protein canopy
• UniProt Gene Name: Cnpy4
• UniProt Synonym Gene Names: Prat4b
• UniProt Entry Name: CNPY4_MOUSE