Cytidine monophosphate-N-acetylneuraminic acid hydroxylase (Cmah) Recombinant Protein | Cmah recombinant protein
Recombinant Mouse Cytidine monophosphate-N-acetylneuraminic acid hydroxylase (Cmah)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Cytidine monophosphate-N-acetylneuraminic acid hydroxylase (Cmah); Recombinant Mouse Cytidine monophosphate-N-acetylneuraminic acid hydroxylase (Cmah); Cmah recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
5-577, Full length protein
Sequence
KQTAETLLTLSPAEVANLKEGINFFRNKTTGKEYILYKEKDHLKACKNLCKHQGGLFMKDIEDLDGRSVKCTKHNWKLDVSTMKYINPPGSFCQDELVIEMDENNGLSLVELNPPNPWDSDPRSPEELAFGEVQITYLTHACMDLKLGDKRMVFDPWLIGPAFARGWWLLHEPPSDWLERLCKADLIYISHMHSDHLSYPTLKQLSQRRPDIPIYVGDTERPVFWNLDQSGVGLTNINVVPFGIWQQVDKSLRFMILMDGVHPEMDTCIIVEYKGHKILNTVDCTRPNGGRLPEKVALMMSDFAGGASGFPMTFSGGKFTEEWKAQFIKAERRKLLNYKAQLVKDLQPRIYCPFAGYFVESHPSDKYIKETNTKNDPNQLNNLIRKNSDVVTWTPRPGAVLDLGRMLKDPTDSKGIVEPPEGTKIYKDSWDFGPYLEILNSAVRDEIFCHSSWIKEYFTWAGFKNYNLVVRMIETDEDFSPFPGGYDYLVDFLDLSFPKERPSREHPYEEIHSRVDVIRYVVKNGLLWDDLYIGFQTRLLRDPDIYHHLFWNHFQIKLPLTPPNWKSFLMHCD
Sequence Length
573
Species
Mouse
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
Related Product Information for Cmah recombinant protein
Sialic acids are terminal components of the carbohydrate chains of glycoconjugates involved in ligand-receptor, cell-cell, and cell-pathogen interactions. The two most common forms of sialic acid found in mammalian cells are N-acetylneuraminic acid (Neu5Ac) and its hydroxylated derivative, N-glycolylneuraminic acid (Neu5Gc). Studies of sialic acid distribution show that Neu5Gc is not detectable in normal human tissues although it was an abundant sialic acid in other mammals. Neu5Gc is, in actuality, immunogenic in humans. The absense of Neu5Gc in humans is due to a deletion within the human gene CMAH encoding cytidine monophosphate-N-acetylneuraminic acid hydroxylase, an enzyme responsible for Neu5Gc biosynthesis. Sequences encoding the mouse, pig, and chimpanzee hydroxylase enzymes were obtained by cDNA cloning and found to be highly homologous. However, the homologous human cDNA differs from these cDNAs by a 92-bp deletion in the 5 region. This deletion, corresponding to exon 6 of the mouse hydroxylase gene, causes a frameshift mutation and premature termination of the polypeptide chain in human. It seems unlikely that the truncated human hydroxylase mRNA encodes for an active enzyme explaining why Neu5Gc is undetectable in normal human tissues. Human genomic DNA also shows evidence of this deletion which does not occur in the genomes of African great apes. Nonetheless, the CMAH gene maps to 6p21.32 in humans and great apes indicating that mutation of the CMAH gene occurred following human divergence from chimpanzees and bonobos.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,151 Da
NCBI Official Full Name
cytidine monophosphate-N-acetylneuraminic acid hydroxylase isoform a
NCBI Official Synonym Full Names
cytidine monophospho-N-acetylneuraminic acid hydroxylase
NCBI Official Symbol
Cmah
NCBI Protein Information
cytidine monophosphate-N-acetylneuraminic acid hydroxylase
UniProt Protein Name
Cytidine monophosphate-N-acetylneuraminic acid hydroxylase
Protein Family
UniProt Gene Name
Cmah
UniProt Synonym Gene Names
CMP-N-acetylneuraminic acid hydroxylase
Uniprot Description
Sialic acids are components of carbohydrate chains of glycoconjugates and are involved in cell-cell recognition and cell-pathogen interactions. Catalyzes the conversion of CMP-N-acetylneuraminic acid (CMP-Neu5Ac) into its hydroxylated derivative CMP-N-glycolylneuraminic acid (CMP-Neu5Gc), a sialic acid abundantly expressed at the surface of many cells.
Research Articles on Cmah
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Product Notes
The Cmah cmah (Catalog #AAA1471334) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 5-577, Full length protein. The amino acid sequence is listed below: KQTAETLLTL SPAEVANLKE GINFFRNKTT GKEYILYKEK DHLKACKNLC KHQGGLFMKD IEDLDGRSVK CTKHNWKLDV STMKYINPPG SFCQDELVIE MDENNGLSLV ELNPPNPWDS DPRSPEELAF GEVQITYLTH ACMDLKLGDK RMVFDPWLIG PAFARGWWLL HEPPSDWLER LCKADLIYIS HMHSDHLSYP TLKQLSQRRP DIPIYVGDTE RPVFWNLDQS GVGLTNINVV PFGIWQQVDK SLRFMILMDG VHPEMDTCII VEYKGHKILN TVDCTRPNGG RLPEKVALMM SDFAGGASGF PMTFSGGKFT EEWKAQFIKA ERRKLLNYKA QLVKDLQPRI YCPFAGYFVE SHPSDKYIKE TNTKNDPNQL NNLIRKNSDV VTWTPRPGAV LDLGRMLKDP TDSKGIVEPP EGTKIYKDSW DFGPYLEILN SAVRDEIFCH SSWIKEYFTW AGFKNYNLVV RMIETDEDFS PFPGGYDYLV DFLDLSFPKE RPSREHPYEE IHSRVDVIRY VVKNGLLWDD LYIGFQTRLL RDPDIYHHLF WNHFQIKLPL TPPNWKSFLM HCD. It is sometimes possible for the material contained within the vial of "Cytidine monophosphate-N-acetylneuraminic acid hydroxylase (Cmah), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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