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Clusterin Recombinant Protein | CLU recombinant protein
Recombinant Clusterin (CLU)
Source: Prokaryotic expression
I SDKELQEMST EGSKYVNKEI KNALKEVKQI KTQIEQTNEE RKLLLSSLEE AKKKKEDALN DTRDSENKLK ASQGVCNETM TALWEECKPC LKQTCMKFYA RVCRSGSGLV GHQLEEFLNQ SSPFYFWING DRIDSLMEND REQSHVMDVM EDSFTRASSI MDELFQDRFF PRRPQDTQYY SPFSSFPRGS LFFNPKSRFA RNVMPFPLLE PLNFHDVFQP FYDMIRQAQQ AMDAHLQRTP YHFPVTEFTE NNDRTVCKEI RHNSTGCLRM KDQCEKCQEI LEVDCSASSP TQTLLRQQLN TSLQLAEKFS RLYDQLLQSY QQKMLDTSTL LKQLNEQFTW VSQLANLTQS DDQYYLQVFT VNSHSSDPSI PSGLTKVVVK LFNSFPITVT VPQEVSSPNF MENVAEKALQ QYRRKSHEE
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
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NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]
Uniprot Description
CLU: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress- induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation. Belongs to the clusterin family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Mitochondrial; Apoptosis; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8p21-p12
Cellular Component: Golgi apparatus; extracellular matrix; extracellular space; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; mitochondrial membrane; extracellular region; nucleus; cytosol
Molecular Function: protein binding; chaperone binding; ubiquitin protein ligase binding; ATPase activity; misfolded protein binding
Biological Process: platelet activation; response to misfolded protein; release of cytochrome c from mitochondria; positive regulation of nitric oxide biosynthetic process; protein stabilization; positive regulation of apoptosis; response to virus; cell morphogenesis; microglial cell activation; positive regulation of tumor necrosis factor production; complement activation; negative regulation of protein homooligomerization; activation of NF-kappaB transcription factor; platelet degranulation; reverse cholesterol transport; myelin maintenance in the central nervous system; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein import; innate immune response; lipid metabolic process; blood coagulation; chaperone-mediated protein complex assembly; complement activation, classical pathway
Research Articles on CLU
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Product Notes
The CLU clu (Catalog #AAA2028888) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Clusterin can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the CLU clu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.I SDKELQEMST EGSKYVNKEI KNALKEVKQI KTQIEQTNEE RKLLLSSLEE AKKKKEDALN DTRDSENKLK ASQGVCNETM TALWEECKPC LKQTCMKFYA RVCRSGSGLV GHQLEEFLNQ SSPFYFWING DRIDSLMEND REQSHVMDVM EDSFTRASSI MDELFQDRFF PRRPQDTQYY SPFSSFPRGS LFFNPKSRFA RNVMPFPLLE PLNFHDVFQP FYDMIRQAQQ AMDAHLQRTP YHFPVTEFTE NNDRTVCKEI RHNSTGCLRM KDQCEKCQEI LEVDCSASSP TQTLLRQQLN TSLQLAEKFS RLYDQLLQSY QQKMLDTSTL LKQLNEQFTW VSQLANLTQS DDQYYLQVFT VNSHSSDPSI PSGLTKVVVK LFNSFPITVT VPQEVSSPNF MENVAEKALQ QYRRKSHEE< /div>. It is sometimes possible for the material contained within the vial of "Clusterin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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