Clusterin ELISA Kit | Clu elisa kit

Rat Clusterin PicoKine ELISA Kit

Cat. Num. AAA1751137

• Gene Names: Clu; CLI; DAG; APOJ; SGP2; SP40; SGP-2; SP-40; Trpm2; Trpmb; TRPM-2; TRPM2B; RATTRPM2B
• Reactivity: Rat; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: Clusterin; Rat Clusterin PicoKine ELISA Kit; Dimeric acid glycoprotein; DAG; Sulfated glycoprotein 2; SGP-2; Testosterone repressed prostate message 2; TRPM-2; Clusterin beta chain; Clusterin alpha chain; Clu; Clu elisa kit

• Reactivity: Rat; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Rat Ces1d
• Sequence Length: 447

• Applicable Applications for Clu elisa kit: ELISA (EIA)
• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta, Citrate)
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <50pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Clu elisa kit

Principle of the Assay: The Picokine Rat Ces1d Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Ces1d with a 96-well strip plate that is pre-coated with antibody specific for Ces1d. The detection antibody is a biotinylated antibody specific for Ces1d. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Rat Ces1d with immunogen: Expression system for standard: NSO, Immunogen sequence: Y19-L565. The kit is analytically validated with ready to use reagents. To measure Rat Ces1d, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Ces1d in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Ces1d in the sample!!Background/Introduction: This gene is mapped to 19p11. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. Enzyme that hydrolizes long-chain fatty acids and thioesters; may play a role in lipid metabolism and/or the elimination of toxic substances. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined.

Product Categories/Family for Clu elisa kit

Immunology; Innate Immunity; complement; classical Pathway; Cardiovascular; Lipids/Lipoproteins; Lipoproteins/Apolipoproteins; Signal Transduction; Protein Trafficking; chaperones; Other chaperones; Metabolism; Lipid Metabolism; Cancer; Tumor Biomarkers

NCBI and Uniprot Product Information

• NCBI GI #: 46048420
• NCBI GeneID: 24854
• NCBI Accession #: NP_444180.2
• NCBI GenBank Nucleotide #: NM_053021.2
• UniProt Accession #: P05371
• Molecular Weight: 51,375 Da
• NCBI Official Full Name: clusterin
• NCBI Official Synonym Full Names: clusterin
• NCBI Official Symbol: Clu
• NCBI Official Synonym Symbols: CLI; DAG; APOJ; SGP2; SP40; SGP-2; SP-40; Trpm2; Trpmb; TRPM-2; TRPM2B; RATTRPM2B
• NCBI Protein Information: clusterin
• UniProt Protein Name: Clusterin
• Protein Family: Clusterin
• UniProt Gene Name: Clu
• UniProt Synonym Gene Names: DAG; SGP-2; TRPM-2

NCBI Description

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders.[provided by RefSeq, May 2011]

Uniprot Description

Functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Promotes apoptosis when in the nucleus. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation ().