Interferon Regulatory Factor 2 Binding Protein 2 (IRF2BP2) CLIA Kit | IRF2BP2 clia kit

CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 2 (IRF2BP2)

Cat. Num. AAA2001060

• Synonyms: Interferon Regulatory Factor 2 Binding Protein 2 (IRF2BP2); CLIA Kit for Interferon Regulatory Factor 2 Binding Protein 2 (IRF2BP2); IRF2BP2 clia kit

• Specificity: This assay has high sensitivity and excellent specificity for detection of Interferon Regulatory Factor 2 Binding Protein 2(IRF2BP2); No significant cross-reactivity or interference between Interferon Regulatory Factor 2 Binding Protein 2(IRF2BP2) and analogues was observed.
• Sequence Length: 587

• Assay Type: Sandwich
• Samples: Tissue homogenates and other biological fluids
• Detection Range: 0.014-10ng/mL The standard curve concentrations used for the ELISA's were 10ng/mL, 3.33ng/mL, 1.11ng/mL, 0.37ng/mL, 0.123ng/mL, 0.041ng/mL, 0.014ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.067ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Regulatory Factor 2 Binding Protein 2(IRF2BP2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Regulatory Factor 2 Binding Protein 2(IRF2BP2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Organism Species: Homo sapiens (Human)
• Assay Length: 4.5 hours
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IRF2BP2 clia kit

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Regulatory Factor 2 Binding Protein 2(IRF2BP2) level in the sample or standard.

NCBI and Uniprot Product Information

• NCBI GI #: 116734704
• NCBI GeneID: 359948
• NCBI Accession #: NP_892017.2
• NCBI GenBank Nucleotide #: NM_182972.2
• UniProt Accession #: Q7Z5L9; Q6P083; Q7Z5L8; Q8N351; Q8WUH8; B1AM35; B1AM36
• Molecular Weight: 61,025 Da
• NCBI Official Full Name: interferon regulatory factor 2-binding protein 2 isoform A
• NCBI Official Synonym Full Names: interferon regulatory factor 2 binding protein 2
• NCBI Official Symbol: IRF2BP2
• NCBI Protein Information: interferon regulatory factor 2-binding protein 2; IRF-2-binding protein 2
• UniProt Protein Name: Interferon regulatory factor 2-binding protein 2
• Protein Family: Interferon regulatory factor 2-binding protein
• UniProt Gene Name: IRF2BP2
• UniProt Synonym Gene Names: IRF-2-binding protein 2; IRF-2BP2
• UniProt Entry Name: I2BP2_HUMAN

NCBI Description

This gene encodes an interferon regulatory factor-2 (IRF2) binding protein that interacts with the C-terminal transcriptional repression domain of IRF2. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

IRF2BP2: Acts as a transcriptional corepressor in a IRF2- dependent manner; this repression is not mediated by histone deacetylase activities. Represses the NFAT1-dependent transactivation of NFAT-responsive promoters. Acts as a coactivator of VEGFA expression in cardiac and skeletal muscle. Belongs to the IRF2BP family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 1q42.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: metal ion binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent