Growth Factor Receptor Bound Protein 10 (Grb10) CLIA Kit | Grb10 clia kit

CLIA Kit for Growth Factor Receptor Bound Protein 10 (Grb10)

Cat. Num. AAA2000769

• Gene Names: GRB10; RSS; IRBP; MEG1; GRB-IR; Grb-10
• Synonyms: Growth Factor Receptor Bound Protein 10 (Grb10); CLIA Kit for Growth Factor Receptor Bound Protein 10 (Grb10); Grb10 clia kit

• Specificity: This assay has high sensitivity and excellent specificity for detection of Growth Factor Receptor Bound Protein 10 (Grb10); No significant cross-reactivity or interference between Growth Factor Receptor Bound Protein 10 (Grb10) and analogues was observed.
• Sequence Length: 536

• Assay Type: Sandwich
• Samples: Tissue homogenates and other biological fluids
• Detection Range: 0.014-10ng/mL The standard curve concentrations used for the ELISA's were 10ng/mL, 3.33ng/mL, 1.11ng/mL, 0.37ng/mL, 0.123ng/mL, 0.041ng/mL, 0.014ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.057ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 10 (Grb10) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Factor Receptor Bound Protein 10 (Grb10) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Organism Species: Rattus norvegicus (Rat)
• Assay Length: 4.5 hours
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Grb10 clia kit

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Factor Receptor Bound Protein 10 (Grb10) level in the sample or standard.

NCBI and Uniprot Product Information

• NCBI GI #: 18999462
• NCBI GeneID: 2887
• UniProt Accession #: Q13322; O00427; O00701; O75222; Q92606; Q92907; Q92948; A4D258; A7VJ95; A8K0E6; D3DVM9
• Molecular Weight: 67,231 Da
• NCBI Official Full Name: Growth factor receptor-bound protein 10
• NCBI Official Synonym Full Names: growth factor receptor-bound protein 10
• NCBI Official Symbol: GRB10
• NCBI Official Synonym Symbols: RSS; IRBP; MEG1; GRB-IR; Grb-10
• NCBI Protein Information: growth factor receptor-bound protein 10; GRB10 adapter protein; GRB10 adaptor protein; maternally expressed gene 1; insulin receptor-binding protein Grb-IR
• UniProt Protein Name: Growth factor receptor-bound protein 10
• Protein Family: Growth factor receptor-bound protein
• UniProt Gene Name: GRB10
• UniProt Synonym Gene Names: GRBIR; KIAA0207
• UniProt Entry Name: GRB10_HUMAN

NCBI Description

The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2010]

Uniprot Description

GRB10: an adaptor protein that inhibits insulin-stimulated IRS-PI (3)K-Akt signaling pathway by disrupting the association of IRS with the insulin receptor. Also binds activated PDGFR and EGFR. Preferentially localizes to the mitochondria, modulating the anti-apoptotic activity of mitochondrial Raf-1. Can translocate to the plasma membrane and lamellipodia. Three alternatively spliced isoforms have been described.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 7p12.2

Cellular Component: cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; SH3/SH2 adaptor activity; insulin receptor binding

Biological Process: negative regulation of glucose import; negative regulation of Wnt receptor signaling pathway; insulin-like growth factor receptor signaling pathway; insulin receptor signaling pathway; negative regulation of insulin receptor signaling pathway; negative regulation of phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of phosphorylation; response to insulin stimulus; negative regulation of glycogen biosynthetic process