CLEC4F recombinant protein
CLEC4F, Recombinant, Mouse (C-type Lectin Domain Family 4 Member F, C-type Lectin Superfamily Member 13, CLECSF13, C-type Lectin 13)
Gene Names
CLEC4F; KCLR; CLECSF13
Reactivity
Mouse
Applications
Western Blot
Purity
Purified85%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin 1EU.
Synonyms
CLEC4F; Recombinant; Mouse (C-type Lectin Domain Family 4 Member F; C-type Lectin Superfamily Member 13; CLECSF13; C-type Lectin 13); CLEC4F recombinant protein
Reactivity
Mouse
Purity/Purification
Purified
85%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin 1EU.
85%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin 1EU.
Form/Format
Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to 0.1mg/ml.
Applicable Applications for CLEC4F recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 55kD migrates as an ~ 65-85kD protein in SDS-PAGE under reducing conditions.
Dilution: Western Blot: 55kD migrates as an ~ 65-85kD protein in SDS-PAGE under reducing conditions.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for CLEC4F recombinant protein
CLEC4F (C-type lectin domain; family 4, member F; also known as the Kupffer cell receptor and fucose receptor) is an 80kD, type II transmembrane glycoprotein member of the C-type lectin superfamily (1-3). Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain (ECD) that contains an extended stalk region plus one carbohydrate recognition domain (4, 5). Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation (6, 7). CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues (8). CLEC4F preferentially binds galactose and N-acetylgalactosamine in a calcium-dependent manner (6, 9, 10). Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system (11).
Product Categories/Family for CLEC4F recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
55kD
NCBI Official Full Name
CLEC4F protein
NCBI Official Synonym Full Names
C-type lectin domain family 4, member F
NCBI Official Symbol
CLEC4F
NCBI Official Synonym Symbols
KCLR; CLECSF13
NCBI Protein Information
C-type lectin domain family 4 member F; C-type lectin 13; C-type lectin superfamily member 13; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 13
UniProt Protein Name
C-type lectin domain family 4 member F
Protein Family
UniProt Gene Name
CLEC4F
UniProt Synonym Gene Names
CLECSF13; C-type lectin 13
UniProt Entry Name
CLC4F_HUMAN
Uniprot Description
CLEC4F: Receptor with an affinity for galactose and fucose. Could be involved in endocytosis.
Protein type: Membrane protein, integral; Extracellular matrix
Chromosomal Location of Human Ortholog: 2p13.3
Cellular Component: plasma membrane; integral to membrane
Molecular Function: galactose binding; glycolipid binding
Biological Process: endocytosis; NK T cell activation
Similar Products
Product Notes
The CLEC4F clec4f (Catalog #AAA638058) is a Recombinant Protein and is intended for research purposes only. The product is available for immediate purchase. The CLEC4F, Recombinant, Mouse (C-type Lectin Domain Family 4 Member F, C-type Lectin Superfamily Member 13, CLECSF13, C-type Lectin 13) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CLEC4F can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 55kD migrates as an ~ 65-85kD protein in SDS-PAGE under reducing conditions. Researchers should empirically determine the suitability of the CLEC4F clec4f for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLEC4F, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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