Human Claudin-2 ELISA Kit | CLDN2 elisa kit

Human Claudin-2 ELISA Kit

Cat. Num. AAA763862

• Reactivity: Human
• Synonyms: Claudin-2; Human Claudin-2 ELISA Kit; CLDN2 (Claudin-2)/SP82; CLDN2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CLDN2. No significant cross-reactivity or interference between CLDN2 and analogues was observed.
• Sequence Length: 230

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CLDN2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 283806634
• NCBI GeneID: 9075
• NCBI Accession #: NP_001164566.1
• NCBI GenBank Nucleotide #: NM_001171095.1
• Molecular Weight: 24,549 Da
• NCBI Official Full Name: claudin-2
• NCBI Official Synonym Full Names: claudin 2
• NCBI Official Symbol: CLDN2
• NCBI Protein Information: claudin-2
• UniProt Protein Name: Claudin-2
• Protein Family: Claudin
• UniProt Gene Name: CLDN2
• UniProt Entry Name: CLD2_HUMAN

NCBI Description

This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.[provided by RefSeq, Jan 2010]

Uniprot Description

Claudin-2: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Cytoskeletal

Chromosomal Location of Human Ortholog: Xq22.3-q23

Cellular Component: integral to membrane; plasma membrane; tight junction

Molecular Function: identical protein binding; protein binding; structural molecule activity

Biological Process: calcium-independent cell-cell adhesion