CLDN18.1/Claudin-18/CLDN18 Recombinant Protein | CLDN18 recombinant protein

Recombinant Human CLDN18.1 /CLDN18 Protein (His Tag)

Cat. Num. AAA2557324

• Gene Names: CLDN18; SFTA5; SFTPJ
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: CLDN18.1/Claudin-18/CLDN18; Recombinant Human CLDN18.1 /CLDN18 Protein (His Tag); Claudin-18; CLDN18; CLDN18 recombinant protein

• Host: E. coli
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 um filtered solution of 25mM Tris-HCl, 25mM NaCl, 0.1% Triton X-100, 10% glycerol, pH 8.0.
• Concentration: 1.27mg/mL. (varies by lot)
• Sequence: Asp28-Leu76

• Species: Human
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Tag: N-His
• Preparation and Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. This product is provided as liquid. It is shipped at frozen temperature with blue ice. Upon receipt, store it immediately at< -20°C.

SDS-PAGE

Related Product Information for CLDN18 recombinant protein

Claudin-18(CLDN18) is a protein that in humans is encoded by the CLDN18 gene. It belongs to the group of claudins. CLDN18 belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells. CLDN18 plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. CLDN18 has two isoform A1 and isoform A2. IMAB362 (Claudiximab) is a monoclonal antibody against isoform 2 of Claudin-18. It is under investigation for the treatment of gastrointestinal adenocarcinomas and pancreatic tumors. IMAB362 was developed by Ganymed Pharmaceuticals AG.

NCBI and Uniprot Product Information

• NCBI GI #: 50345279
• NCBI GeneID: 51208
• NCBI Accession #: NP_001002026.1
• NCBI GenBank Nucleotide #: NP_001002026.1
• UniProt Accession #: P56856
• Molecular Weight: Molecular Mass: 19.8kDa; Actual Protein Molecular Mass: 18kDa
• NCBI Official Full Name: claudin-18 isoform 2
• NCBI Official Synonym Full Names: claudin 18
• NCBI Official Symbol: CLDN18
• NCBI Official Synonym Symbols: SFTA5; SFTPJ
• NCBI Protein Information: claudin-18
• UniProt Protein Name: Claudin-18
• Protein Family: Claudin
• UniProt Gene Name: CLDN18
• UniProt Entry Name: CLD18_HUMAN

NCBI Description

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is upregulated in patients with ulcerative colitis and highly overexpressed in infiltrating ductal adenocarcinomas. PKC/MAPK/AP-1 (protein kinase C/mitogen-activated protein kinase/activator protein-1) dependent pathway regulates the expression of this gene in gastric cells. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jun 2010]

Uniprot Description

Claudin-18: a multi-pass membrane protein with calcium-independent cell-adhesion activity that plays a major role in the formation of tight junctions. Belongs to the claudin family. Two isoforms of the human protein are produced by alternative splicing. Isoform 2 is a specific cell lineage marker. Its expression in normal tissues is restricted to differentiated epithelial cells of the gastric mucosa; it is absent from the gastric stem cells. Isoform 2 is expressed in a significant proportion of primary gastric cancers and metastases, as well as in subtypes of pancreatic, esophageal, ovarian, and lung tumors.

Protein type: Cell adhesion; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q22.3

Cellular Component: tight junction; integral to membrane; plasma membrane

Molecular Function: identical protein binding; structural molecule activity

Biological Process: intercellular junction assembly and maintenance; calcium-independent cell-cell adhesion