Claudin 1 Antibody | anti-CLDN1 antibody

Claudin 1 Antibody

Cat. Num. AAA853984

• Gene Names: CLDN1; CLD1; SEMP1; ILVASC
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: Claudin 1; Claudin 1 Antibody; CLD1; SEMP1; Senescence-associated epithelial membrane protein; anti-CLDN1 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: Claudin 1 antibody detects endogenous levels of total Claudin 1 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 211

• Applicable Applications for anti-CLDN1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:5000
• Immunogen: The antiserum was produced against synthesized peptide derived from human Claudin 1.
• Preparation and Storage: Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Claudin 1 antibody.)

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells, using Claudin 1 antibody.)

References

Julie Dufresne and Daniel G. Cyr, Biol Reprod, May 2007; 76: 825-832. Evemie Dubé, Biol Reprod, Apr 2007; 10.1095/biolreprod.106.059246. Michele A. Battle, PNAS, May 2006; 103: 8419-8424. Edmund C. Chang, Endocrinology, Oct 2006; 147: 4831-4842.

NCBI and Uniprot Product Information

• NCBI GI #: 10863887
• NCBI GeneID: 9076
• NCBI Accession #: NP_066924.1
• NCBI GenBank Nucleotide #: NM_021101.4
• UniProt Accession #: O95832
• Molecular Weight: 22,744 Da
• NCBI Official Full Name: claudin-1
• NCBI Official Synonym Full Names: claudin 1
• NCBI Official Symbol: CLDN1
• NCBI Official Synonym Symbols: CLD1; SEMP1; ILVASC
• NCBI Protein Information: claudin-1
• UniProt Protein Name: Claudin-1
• Protein Family: Claudin
• UniProt Gene Name: CLDN1
• UniProt Synonym Gene Names: CLD1; SEMP1

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).