Creatine Kinase Recombinant Protein | CKMT2 recombinant protein

Recombinant Creatine Kinase, Mitochondrial 2, Sarcomeric (CKMT2)

Cat. Num. AAA2030997

• Gene Names: CKMT1B; CKMT; CKMT1; UMTCK
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Creatine Kinase; Recombinant Creatine Kinase; Mitochondrial 2; Sarcomeric (CKMT2); CKMT2 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; E VREQPRLFPPSADYPDLRKH NNCMAECLTP AIYAKLRNKV TPNGYTLDQC IQTGVDNPGHPFIKTVGMVA GDEESYEVFA DLFDPVIKLR HNGYDPRVMK HTTDLDASKITQGQFDEHYV LSSRVRTGRS IRGLSLPPAC TRAERREVEN VAITALEGLKGDLAGRYYKL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG MARDWPDARGIWHNYDKTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQERGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLRLQKRGTGGVD TAAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKKLERGQDIKVP PPLPQFGKK
• Sequence Length: 417

• Applicable Applications for CKMT2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 47.0kDa
• Accurate Molecular Mass (KD): 47kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Homo sapiens (Human)
• Fragment: Glu40~Lys419
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 180590
• NCBI GeneID: 1159
• Molecular Weight: 50,421 Da
• NCBI Official Full Name: creatine kinase
• NCBI Official Synonym Full Names: creatine kinase, mitochondrial 1B
• NCBI Official Symbol: CKMT1B
• NCBI Official Synonym Symbols: CKMT; CKMT1; UMTCK
• NCBI Protein Information: creatine kinase U-type, mitochondrial
• UniProt Protein Name: Creatine kinase U-type, mitochondrial
• Protein Family: Creatine kinase
• UniProt Gene Name: CKMT1A
• UniProt Synonym Gene Names: CKMT; Mia-CK; U-MtCK
• UniProt Entry Name: KCRU_HUMAN

NCBI Description

Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Many malignant cancers with poor prognosis have shown overexpression of ubiquitous mitochondrial creatine kinase; this may be related to high energy turnover and failure to eliminate cancer cells via apoptosis. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase. Two genes located near each other on chromosome 15 have been identified which encode identical mitochondrial creatine kinase proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

CKMT1A: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family. Exists as an octamer composed of four MTCK homodimers. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, other; Amino Acid Metabolism - arginine and proline; Mitochondrial; EC 2.7.3.2

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: creatine kinase activity; ATP binding

Biological Process: phosphorylation; creatine metabolic process