Chicken Chemokine Like Factor Superfamily 5 ELISA Kit | CKLFSF5 elisa kit

Chicken Chemokine Like Factor Superfamily 5 ELISA Kit

Cat. Num. AAA745218

• Gene Names: CMTM5; CKLFSF5
• Reactivity: Chicken
• Synonyms: Chemokine Like Factor Superfamily 5; Chicken Chemokine Like Factor Superfamily 5 ELISA Kit; CKLFSF5 elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This CKLFSF5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CKLFSF5. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CKLFSF5 elisa kit

Principle of the assay: CKLFSF5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CKLFSF5 antibody and an CKLFSF5-HRP conjugate. The assay sample and buffer are incubated together with CKLFSF5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CKLFSF5 concentration since CKLFSF5 from samples and CKLFSF5-HRP conjugate compete for the anti-CKLFSF5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CKLFSF5 from the sample, fewer sites are left to bind CKLFSF5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CKLFSF5 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CKLFSF5 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 82546841
• NCBI GeneID: 116173
• NCBI Accession #: NP_001032365.1
• NCBI GenBank Nucleotide #: NM_001037288.1
• Molecular Weight: 14,218 Da
• NCBI Official Full Name: CKLF-like MARVEL transmembrane domain-containing protein 5 isoform c
• NCBI Official Synonym Full Names: CKLF-like MARVEL transmembrane domain containing 5
• NCBI Official Symbol: CMTM5
• NCBI Official Synonym Symbols: CKLFSF5
• NCBI Protein Information: CKLF-like MARVEL transmembrane domain-containing protein 5; chemokine-like factor super family 5; chemokine-like factor superfamily member 5
• UniProt Protein Name: CKLF-like MARVEL transmembrane domain-containing protein 5
• UniProt Gene Name: CMTM5
• UniProt Synonym Gene Names: CKLFSF5
• UniProt Entry Name: CKLF5_HUMAN

NCBI Description

This gene encodes a member of the chemokine-like factor superfamily. This family of genes encodes multi-pass membrane proteins that are similar to both the chemokine and the transmembrane 4 superfamilies of signaling molecules. The encoded protein may exhibit tumor suppressor activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

CMTM5: a member of the chemokine-like factor gene superfamily. This family of genes encodes multi-pass membrane proteins that are similar to both the chemokine and the transmembrane 4 superfamilies of signaling molecules. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space; integral to membrane

Molecular Function: cytokine activity

Biological Process: chemotaxis; negative regulation of myoblast differentiation